Single-cell printing based on impedance detection

Biomicrofluidics
J SchoendubePeter Koltay

Abstract

Label-free isolation of single cells is essential for the growing field of single-cell analysis. Here, we present a device which prints single living cells encapsulated in free-flying picoliter droplets. It combines inkjet printing and impedance flow cytometry. Droplet volume can be controlled in the range of 500 pl-800 pl by piezo actuator displacement. Two sets of parallel facing electrodes in a 50 μm × 55 μm channel are applied to measure the presence and velocity of a single cell in real-time. Polystyrene beads with <5% variation in diameter generated signal variations of 12%-17% coefficients of variation. Single bead efficiency (i.e., printing events with single beads vs. total number of printing events) was 73% ± 11% at a throughput of approximately 9 events/min. Viability of printed HeLa cells and human primary fibroblasts was demonstrated by culturing cells for at least eight days.

References

Aug 31, 2006·Biotechnology Journal·Thomas BolandXiaofeng Cui
Jun 29, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Karen C CheungAttila Tárnok
Mar 2, 2011·Lab on a Chip·Daniel Spencer, Hywel Morgan
Mar 15, 2011·ACS Combinatorial Science·Albert R LiberskiUlrich S Schubert
Mar 18, 2011·PloS One·Sangjun MoonUtkan Demirci
Oct 1, 2011·Biotechnology and Bioengineering·F DavidM Di Berardino
Nov 15, 2011·Nature Biotechnology·Piero DalerbaStephen R Quake
Mar 1, 2012·Asian Cardiovascular & Thoracic Annals·Aron-Frederik PopovTomislav Stojanovic
Apr 4, 2012·Annual Review of Chemical and Biomolecular Engineering·Frederik S O FritzschAndreas Schmid
Jul 31, 2013·Nature Reviews. Genetics·Ehud ShapiroSten Linnarsson
Nov 14, 2013·Journal of Laboratory Automation·Andre GrossPeter Koltay
Jan 10, 2014·Biomicrofluidics·Lin FengFumihito Arai
Jan 11, 2014·Lab on a Chip·Yuchao ChenTony Jun Huang
Feb 14, 2014·Nature Methods

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Citations

Nov 7, 2018·Lab on a Chip·Aude I SegalinyWeian Zhao
Dec 12, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ning MaMichelle A Digman
Jun 5, 2018·Biomicrofluidics·Jinzhen FanTingrui Pan
May 26, 2018·The Analyst·Hyungseok ChoKi-Ho Han
May 23, 2019·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·Aihui WangXianting Ding
Aug 12, 2021·Biosensors & Bioelectronics·Tao TangYaxiaer Yalikun

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