Single-cell quantitative RT-PCR analysis of Cpt1b and Cpt2 gene expression in mouse antral oocytes and in preimplantation embryos

Cytogenetic and Genome Research
L GentileMaurizio Zuccotti

Abstract

Fatty acids represent an important energy source for preimplantation embryos. Fatty acids oxidation is correlated with the embryo oxygen consumption which remains relatively constant up to the 8-cell stage, but suddenly increases between the 8-cell and morula stages. The degradation of fatty acids occurs in mitochondria and is catalyzed by several carnitine acyl transferases, including two carnitine palmitoyl transferases, CPT-I and CPT-II. We have carried out a study to determine the relative number of transcripts of Cpt1b and Cpt2 genes encoding for m-CPT-I and CPT-II enzymes, during mouse preimplantation development. Here we show that Cpt1b transcripts are first and temporally detected at the 2-cell stage and reappear at the morula and blastocyst stage. Cpt2 transcripts decrease following fertilization to undetectable levels and are present again later at the morula stage. These results show that transcription of both Cpt1b and Cpt2 is triggered at the morula stage, concomitantly with known increasing profiles of oxygen uptake and fatty acids oxidation. Based on the number of Cpt2 transcripts detected, we could discriminate the presence of two groups of embryos with high and low number of transcripts, from the zygote through...Continue Reading

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Citations

Apr 20, 2005·Molecular and Cellular Endocrinology·Maurizio ZuccottiCarlo Alberto Redi
Apr 25, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Kylie R DunningRebecca L Robker
Nov 21, 2007·Molecular Genetics and Metabolism·Shaonin JiPhilip A Wood
Dec 1, 2004·Molecular Reproduction and Development·Vittorio SebastianoMaurizio Zuccotti
Feb 24, 2006·Molecular Reproduction and Development·Manuela MontiMaurizio Zuccotti
Oct 5, 2014·Cellular and Molecular Life Sciences : CMLS·Ling GuQiang Wang
Feb 27, 2016·Molecular Human Reproduction·Michele Boiani, Jose B Cibelli
Jul 20, 2017·Molecular Reproduction and Development·Kailun Hu, Yang Yu
Mar 10, 2016·Asian-Australasian Journal of Animal Sciences·Seung-Hun KimChang-Kyu Lee

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