Single-cell recording and stimulation with a 16k micro-nail electrode array integrated on a 0.18 μm CMOS chip

Lab on a Chip
Roeland HuysWolfgang Eberle

Abstract

To cope with the growing needs in research towards the understanding of cellular function and network dynamics, advanced micro-electrode arrays (MEAs) based on integrated complementary metal oxide semiconductor (CMOS) circuits have been increasingly reported. Although such arrays contain a large number of sensors for recording and/or stimulation, the size of the electrodes on these chips are often larger than a typical mammalian cell. Therefore, true single-cell recording and stimulation remains challenging. Single-cell resolution can be obtained by decreasing the size of the electrodes, which inherently increases the characteristic impedance and noise. Here, we present an array of 16,384 active sensors monolithically integrated on chip, realized in 0.18 μm CMOS technology for recording and stimulation of individual cells. Successful recording of electrical activity of cardiac cells with the chip, validated with intracellular whole-cell patch clamp recordings are presented, illustrating single-cell readout capability. Further, by applying a single-electrode stimulation protocol, we could pace individual cardiac cells, demonstrating single-cell addressability. This novel electrode array could help pave the way towards solving co...Continue Reading

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Citations

Feb 6, 2013·Nature Nanotechnology·Micha E Spira, Aviad Hai
Jun 21, 2013·PloS One·Liesbeth MicholtCarmen Bartic
Apr 1, 2014·Lab on a Chip·Timir Datta-ChaudhuriElisabeth Smela
Aug 30, 2012·Lab on a Chip·Dries BraekenGeert Callewaert
Feb 3, 2016·Scientific Reports·Marleen WelkenhuysenDries Braeken
Dec 23, 2015·Chemical Reviews·Anqi Zhang, Charles M Lieber
Apr 19, 2015·Current Opinion in Neurobiology·Matthew R AngleNicholas A Melosh
Jan 23, 2015·Frontiers in Neuroscience·Marie Engelene J ObienUrs Frey
Jul 5, 2016·Frontiers in Neuroscience·Jeffrey R NaughtonMichael J Naughton
Jan 6, 2017·IEEE Transactions on Biomedical Circuits and Systems·Timir Datta-ChaudhuriPamela A Abshire
Oct 28, 2017·European Biophysics Journal : EBJ·Viktorija RadotićDamir Kovačić
Nov 9, 2016·Reports on Progress in Physics·Wei ZhouCharles M Lieber
May 24, 2019·Frontiers in Cellular Neuroscience·Vishalini EmmeneggerAndreas Hierlemann
Jun 3, 2017·The Review of Scientific Instruments·Limin SunMatti Hämäläinen
Nov 1, 2017·Advanced Biosystems·Günther ZeckRoland Thewes
Feb 14, 2017·Nature Nanotechnology·Jeffrey AbbottHongkun Park
May 21, 2019·Frontiers in Neuroscience·Vijay ViswamAndreas Hierlemann
Oct 12, 2018·Sensors·Joni KilpijärviAnita Lloyd Spetz
Jan 28, 2021·Micromachines·Csaba ForroGabriella Panuccio
Apr 3, 2021·Journal of Nanobiotechnology·Jia GaoGuibin Jiang
May 21, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pranay RanjanPrashant Kumar
Jul 31, 2021·Frontiers in Neuroscience·Norberto Pérez-Prieto, Manuel Delgado-Restituto
Feb 14, 2018·Accounts of Chemical Research·Jeffrey AbbottHongkun Park
Sep 29, 2021·Chemical Reviews·Anna MarianoFrancesca Santoro

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