Single-cell resolution of intracellular T cell Ca2+ dynamics in response to frequency-based H2 O2 stimulation

Integrative Biology : Quantitative Biosciences From Nano to Macro
Ariel S Kniss-JamesMelissa L Kemp


Adaptive immune cells, such as T cells, integrate information from their extracellular environment through complex signaling networks with exquisite sensitivity in order to direct decisions on proliferation, apoptosis, and cytokine production. These signaling networks are reliant on the interplay between finely tuned secondary messengers, such as Ca2+ and H2O2. Frequency response analysis, originally developed in control engineering, is a tool used for discerning complex networks. This analytical technique has been shown to be useful for understanding biological systems and facilitates identification of the dominant behaviour of the system. We probed intracellular Ca2+ dynamics in the frequency domain to investigate the complex relationship between two second messenger signaling molecules, H2O2 and Ca2+, during T cell activation with single cell resolution. Single-cell analysis provides a unique platform for interrogating and monitoring cellular processes of interest. We utilized a previously developed microfluidic device to monitor individual T cells through time while applying a dynamic input to reveal a natural frequency of the system at approximately 2.78 mHz stimulation. Although our network was much larger with more unkno...Continue Reading


Mar 13, 2001·Annual Review of Immunology·R S Lewis
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Jul 26, 2003·The EMBO Journal·Taichiro TomidaMasamitsu Iino
Sep 25, 2003·Biochemical Society Transactions·R S Lewis
Jan 28, 2006·Redox Report : Communications in Free Radical Research·Helen R Griffiths
Jul 3, 2007·Free Radical Biology & Medicine·J David LambethBecky Diebold
Sep 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard J DiPaoloEthan M Shevach
Dec 11, 2007·Biophysical Journal·Alexander SkupinMartin Falcke
Jan 26, 2008·Science·Jerome T MettetalAlexander van Oudenaarden
Feb 19, 2008·Bio Systems·Johannes MüllerBurkhard A Hense
Aug 1, 2008·Nature·Matthew R BennettJeff Hasty
Aug 30, 2008·Blood·Jinfang Zhu, William E Paul
Sep 13, 2008·Current Biology : CB·Michael J Boulware, Jonathan S Marchant
Jan 6, 2009·Nature Methods·Alison M SkelleyJoel Voldman
Jun 17, 2009·Biochimica Et Biophysica Acta·György Csordás, György Hajnóczky
Mar 3, 2010·Annual Review of Immunology·Jinfang ZhuWilliam E Paul
Mar 20, 2010·Lab on a Chip·Stefan KobelMatthias Lutolf
Sep 3, 2010·Trends in Biochemical Sciences·Anant B Parekh
Dec 28, 2010·Nature Methods·Kwanghun ChungHang Lu
Mar 19, 2011·Cell·Gábor BalázsiJames J Collins
Sep 24, 2011·Antioxidants & Redox Signaling·Gaurav Dwivedi, Melissa L Kemp
Jun 16, 2012·Nature Reviews. Immunology·Stefan FeskeMurali Prakriya
Aug 21, 2012·Journal of Molecular and Cellular Cardiology·Gerald W Dorn, Christoph Maack
Sep 4, 2012·Antioxidants & Redox Signaling·Pravin KesarwaniShikhar Mehrotra
Dec 6, 2012·Journal of Mathematical Physics·Likun ZhengQing Nie
Jun 5, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Andreja JovicShuichi Takayama
Nov 26, 2013·Biochimica Et Biophysica Acta·Erik Smedler, Per Uhlén
Nov 13, 2014·Frontiers in Genetics·Magali Richard, Gaël Yvert
Jul 4, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ramesh RamjiKathryn Miller-Jensen

❮ Previous
Next ❯


Jan 13, 2018·Annual Review of Biomedical Engineering·Pamela K KreegerKristyn S Masters
Aug 10, 2017·Analytical Chemistry·Peng ChenBi-Feng Liu
Nov 9, 2018·Analytical Chemistry·Gongchen Sun, Hang Lu
Aug 30, 2021·Biophysical Journal·Cecilia VillarruelSilvina Ponce Dawson

❮ Previous
Next ❯

Related Concepts

Related Feeds


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.