Single-cell resolution of intracellular T cell Ca2+ dynamics in response to frequency-based H2 O2 stimulation

Integrative Biology : Quantitative Biosciences From Nano to Macro
Ariel S Kniss-JamesMelissa L Kemp

Abstract

Adaptive immune cells, such as T cells, integrate information from their extracellular environment through complex signaling networks with exquisite sensitivity in order to direct decisions on proliferation, apoptosis, and cytokine production. These signaling networks are reliant on the interplay between finely tuned secondary messengers, such as Ca2+ and H2O2. Frequency response analysis, originally developed in control engineering, is a tool used for discerning complex networks. This analytical technique has been shown to be useful for understanding biological systems and facilitates identification of the dominant behaviour of the system. We probed intracellular Ca2+ dynamics in the frequency domain to investigate the complex relationship between two second messenger signaling molecules, H2O2 and Ca2+, during T cell activation with single cell resolution. Single-cell analysis provides a unique platform for interrogating and monitoring cellular processes of interest. We utilized a previously developed microfluidic device to monitor individual T cells through time while applying a dynamic input to reveal a natural frequency of the system at approximately 2.78 mHz stimulation. Although our network was much larger with more unkno...Continue Reading

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Citations

Jan 13, 2018·Annual Review of Biomedical Engineering·Pamela K KreegerKristyn S Masters
Aug 10, 2017·Analytical Chemistry·Peng ChenBi-Feng Liu
Nov 9, 2018·Analytical Chemistry·Gongchen Sun, Hang Lu
Aug 30, 2021·Biophysical Journal·Cecilia VillarruelSilvina Ponce Dawson

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