Single-Cell RNA Analysis of Type I Spiral Ganglion Neurons Reveals a Lmx1a Population in the Cochlea.

Frontiers in Molecular Neuroscience
Fiorella Carla GrandiM Mustapha

Abstract

In the mature cochlea, each inner hair cell (IHC) is innervated by multiple spiral ganglion neurons of type I (SGNI). SGNIs are morphologically and electro-physiologically diverse. Also, they differ in their susceptibility to noise insult. However, the molecular underpinnings of their identity and physiological differences remain poorly understood. In this study, we developed a novel triple transgenic mouse, which enabled the isolation of pure populations of SGNIs and the analysis of a 96-gene panel via single-cell qPCR. We found three distinct populations of Type I SGNs, which were marked by their exclusive expression of Lmx1a, Slc4a4, or Mfap4/Fzd2, respectively, at postnatal days P3, P8, and P12. Our data suggest that afferent SGN subtypes are established genetically before the onset of hearing and that the expression of key physiological markers, such as ion channels, is heterogeneous and may be underlying the heterogeneous firing proprieties of SGNIs.

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Citations

Jul 3, 2021·Frontiers in Cellular Neuroscience·Karen L ElliottEbenezer N Yamoah
Aug 20, 2021·Current Opinion in Otolaryngology & Head and Neck Surgery·Shuohao SunUlrich Müller

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Methods Mentioned

BETA
transgenic
fluorescence-activated cell sorting
Fluorescence-Activated
FACS
PCR
chip
PCA
cochlear section

Software Mentioned

Smart
Prism Graphpad
Fluidigm
Python
Velocity
seq2
HDBSCAN

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