DOI: 10.1101/469346Nov 13, 2018Paper

Single-cell RNA-seq of the Developing Cardiac Outflow Tract Reveals Convergent Development of the Vascular Smooth Muscle Cells at the Base of the Great Arteries

BioRxiv : the Preprint Server for Biology
Xuanyu LiuZhou Zhou

Abstract

Rationale: Cardiac outflow tract (OFT) is a major hotspot for congenital heart diseases (CHDs). A thorough understanding of the cellular diversity, transitions and regulatory networks of normal OFT development is essential to decipher the etiology of OFT malformations. Objective: We sought to explore the cellular diversity and transitions between cell lineages during OFT development. Methods and Results: We performed single-cell transcriptomic sequencing of 55,611 mouse OFT cells from three developmental stages that generally correspond to the early, middle and late stages of OFT remodeling and septation. We identified 17 cell clusters that could be assigned to six cell lineages. Among these lineages, the macrophage and VSMC lineages of the developing OFT have seldom been previously described. Known cellular transitions, such as endothelial to mesenchymal transition, have been recapitulated. In particular, we identified convergent development of the VSMC lineage, where intermediate cell subpopulations were found to be involved in either myocardial to VSMC trans-differentiation or mesenchymal to VSMC transition. Through single-molecule in situ hybridization, we observed that cells expressing the myocardial marker Myh7 co-express...Continue Reading

Related Concepts

Arteries
Cell Differentiation Process
Connective Tissue Cells
Gene Clusters
Heart
Macrophage
Laboratory mice
Myocardium
Mesenchyma
In Situ Hybridization

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