Single-cell RNA sequencing reveals a signature of sexual commitment in malaria parasites

Nature
Asaf PoranBjörn F C Kafsack

Abstract

Pathogens have to balance transmission with persistence. For Plasmodium falciparum, the most widespread and virulent malaria parasite, persistence within its human host requires continuous asexual replication within red blood cells, while its mosquito-borne transmission depends on intra-erythrocytic differentiation into non-replicating sexual stages called gametocytes. Commitment to either fate is determined during the preceding cell cycle that begins with invasion by a single, asexually committed merozoite and ends, 48 hours later, with a schizont releasing newly formed merozoites, all committed to either continued asexual replication or differentiation into gametocytes. Sexual commitment requires the transcriptional activation of ap2-g (PF3D7_1222600), the master regulator of sexual development, from an epigenetically silenced state during asexual replication. AP2-G expression during this 'commitment cycle' prepares gene expression in nascent merozoites to initiate sexual development through a hitherto unknown mechanism. To maintain a persistent infection, the expression of ap2-g is limited to a sub-population of parasites (1-30%, depending on genetic background and growth conditions). As sexually committed schizonts comprise...Continue Reading

References

Nov 5, 1999·Trends in Biochemical Sciences·M Laurent, N Kellershohn
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 5, 2006·Behavior Research Methods·Sunil Subrao Pai, Francis T Durso
Sep 19, 2006·PLoS Computational Biology·Vijay ChickarmaneCarsten Peterson
May 25, 2007·Molecular and Biochemical Parasitology·Quinton L FivelmanDavid A Baker
Nov 13, 2007·Nature Methods·Christopher M Armstrong, Daniel E Goldberg
Sep 22, 2009·International Journal for Parasitology·Jennifer VolzAlan F Cowman
Feb 4, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Sudin BhattacharyaQiang Zhang
Jul 27, 2010·Bioinformatics·Christian T LopesGary D Bader
Jan 29, 2013·Cellular Microbiology·Julien Guizetti, Artur Scherf
Aug 6, 2013·Cell·Geeta J NarlikarTom Owen-Hughes
Aug 15, 2014·Cell Host & Microbe·Nicolas M B BrancucciTill S Voss
Aug 15, 2014·Cell Host & Microbe·Bradley I ColemanManoj T Duraisingh
Apr 14, 2015·Nature Biotechnology·Rahul SatijaAviv Regev
May 23, 2015·Cellular Microbiology·Karell G PelléMatthias Marti
Oct 22, 2016·Cell·Alan F CowmanKevin Marsh
Jan 24, 2017·Nature Methods·Xiaojie QiuCole Trapnell
Jan 25, 2017·Scientific Reports·Antoine ClaessensDavid J Conway

❮ Previous
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Citations

Dec 5, 2017·Frontiers in Microbiology·Meghan ZuckAlexis Kaushansky
Jun 22, 2018·Annual Review of Microbiology·Manoj T Duraisingh, Kristen M Skillman
Jun 23, 2018·Annual Review of Microbiology·Kami Kim
Jul 6, 2018·Annual Review of Microbiology·Gabrielle A JoslingManuel Llinás
Sep 8, 2018·Annual Review of Genetics·Kenneth D Birnbaum
Jul 17, 2018·Parasitology·Divya BeriUtpal Tatu
May 23, 2019·Briefings in Functional Genomics·Oriol Llorà-BatlleAlfred Cortés
Nov 8, 2018·Nature Communications·Pratip K ChattopadhyayDiane L Bolton
Nov 15, 2018·PLoS Pathogens·Petra SchneiderSarah E Reece
Jun 21, 2019·Briefings in Functional Genomics·Christa G Toenhake, Richárd Bártfai
Dec 5, 2019·BMC Genomics·Riëtte van BiljonLyn-Marie Birkholtz
Oct 8, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Chuan Ku, Arnau Sebé-Pedrós
Jan 11, 2020·Nature Reviews. Microbiology·Kannan VenugopalMatthias Marti
Sep 26, 2019·Malaria Journal·Reto CaldelariVolker Heussler
May 7, 2019·Briefings in Functional Genomics·José L Ruiz, Elena Gómez-Díaz
Mar 22, 2020·Nature Communications·Gabrielle A JoslingManuel Llinás
Apr 27, 2018·Microbiology and Molecular Biology Reviews : MMBR·Hyun Jae LeeAubrey J Cunnington
Jun 21, 2019·FEMS Microbiology Reviews·Priscilla NgothoMatthias Marti
Nov 1, 2018·Wellcome Open Research·Nicolas M B BrancucciMatthias Marti
Apr 10, 2019·Pathogens·Myriam D JeningaMichaela Petter
Nov 28, 2018·Nature Microbiology·Cristina BancellsAlfred Cortés
Oct 30, 2019·Evolution, Medicine, and Public Health·Philip L G BirgetSarah E Reece
Nov 26, 2020·Molecular Microbiology·Manish KumarManoj T Duraisingh
Feb 16, 2021·Frontiers in Cellular and Infection Microbiology·Annemarie Voorberg-van der WelAnne-Marie Zeeman

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