Single-cell RNA sequencing reveals distinct gene expression patterns in glucose metabolism of human preimplantation embryos

Reproduction, Fertility, and Development
Di-Cheng ZhaoLing-Qian Wu

Abstract

Precise regulation of glucose metabolism-related genes is essential for early embryonic development. Although previous research has yielded detailed information on the biochemical processes, little is yet known of the dynamic gene expression profiles in glucose metabolism of preimplantation embryos at a single-cell resolution. In the present study, we performed integrated analysis of single-cell RNA sequencing (scRNA-seq) data of human preimplantation embryos that had been cultured in sequential medium. Different cells in the same embryo have similar gene expression patterns in glucose metabolism. During the switch from the cleavage to morula stage, the expression of glycolysis-related genes, such as glucose transporter genes (solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1) and solute carrier family 2 (facilitated glucose transporter), member 3 (SLC2A3) and genes encoding hexokinase, phosphofructokinase, pyruvate kinase and lactate dehydrogenase, is increased. The genes involved in the pentose phosphate pathway are highly expressed at the cleavage stage, generating the reducing power to balance oxidative stress derived from biosynthesis. Expression of the genes involved in the biosynthesis of glycer...Continue Reading

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Citations

Aug 10, 2019·Journal of Assisted Reproduction and Genetics·Nilay KuscuCiler Celik-Ozenci
Apr 10, 2020·Viruses·Claudia CavaIsabella Castiglioni

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