Single-cell RNA sequencing reveals the cellular heterogeneity of aneurysmal infrarenal abdominal aorta.

Cardiovascular Research
Guizhen ZhaoJifeng Zhang

Abstract

The artery contains numerous cell types which contribute to multiple vascular diseases. However, the heterogeneity and cellular responses of these vascular cells during abdominal aortic aneurysm (AAA) progression have not been well characterized. Single-cell RNA sequencing was performed on the infrarenal abdominal aortas (IAAs) from C57BL/6J mice at Days 7 and 14 post-sham or peri-adventitial elastase-induced AAA. Unbiased clustering analysis of the transcriptional profiles from >4500 aortic cells identified 17 clusters representing nine-cell lineages, encompassing vascular smooth muscle cells (VSMCs), fibroblasts, endothelial cells, immune cells (macrophages, T cells, B cells, and dendritic cells), and two types of rare cells, including neural cells and erythrocyte cells. Seurat clustering analysis identified four smooth muscle cell (SMC) subpopulations and five monocyte/macrophage subpopulations, with distinct transcriptional profiles. During AAA progression, three major SMC subpopulations were proportionally decreased, whereas the small subpopulation was increased, accompanied with down-regulation of SMC contractile markers and up-regulation of pro-inflammatory genes. Another AAA-associated cellular response is immune cell e...Continue Reading

References

Mar 10, 2000·Journal of Vascular Surgery·V L RoweM H Goldman
Mar 5, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Mitsuru AbeToru Kita
Nov 17, 2004·Genes & Development·Valérie DomengaAnne Joutel
Dec 14, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·John J LeporeMichael S Parmacek
Nov 22, 2005·Clinical and Experimental Immunology·C GalleM Goldman
Feb 25, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Koichi ShimizuPeter Libby
Dec 8, 2007·The Journal of Biological Chemistry·Jinghai WuJerry B Lingrel
Aug 20, 2009·Cardiology in Review·Konstantinos D RizasMartin D Tilson
Nov 26, 2009·The Journal of Thoracic and Cardiovascular Surgery·Gorav AilawadiGary K Owens
Nov 17, 2010·Nature Reviews. Cardiology·Ian M NordonMatt M Thompson
Jul 14, 2011·Journal of Visualized Experiments : JoVE·Matthew J ButcherElena Galkina
Aug 6, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Jiusong SunGuo-Ping Shi
Jul 26, 2012·Surgery·Castigliano M BhamidipatiGorav Ailawadi
May 4, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Chiara BolegoGiulia Chinetti-Gbaguidi
Jan 17, 2015·Circulation Research·Peter Libby, Göran K Hansson
Apr 11, 2015·Circulation Research·Peter J Psaltis, Robert D Simari
Jan 20, 2016·Nature Immunology·Kay KlapprothHans-Reimer Rodewald
Jul 28, 2016·Circulation Research·Matthias Nahrendorf, Filip K Swirski
Aug 21, 2016·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·J Lysgaard PoulsenJ S Lindholt
Mar 10, 2017·Journal of Vascular Surgery·Guanyi LuGilbert R Upchurch
Apr 14, 2017·Nature Reviews. Cardiology·Juliette RaffortZiad Mallat
Apr 18, 2017·Progress in Molecular Biology and Translational Science·Simon W Rabkin
Aug 9, 2017·Nature Reviews. Immunology·Efthymia Papalexi, Rahul Satija
Jan 6, 2018·Circulation Research·Lisa Honold, Matthias Nahrendorf
Feb 24, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Akshaya K MeherGilbert R Upchurch
Dec 7, 2018·Nature·Brent A BiddySamantha A Morris
Feb 28, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Frank M DavisHong S Lu
Apr 5, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Wenduo GuQingbo Xu
May 31, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Ploingarm PetsophonsakulLeon Schurgers

❮ Previous
Next ❯

Citations

Feb 23, 2021·Frontiers in Immunology·Zhen YuanZhejun Cai
May 25, 2021·Circulation·Joseph M MianoMark W Majesky
Jun 29, 2021·Frontiers in Cardiovascular Medicine·Mengxia Fu, Jiangping Song
Jun 29, 2021·Cardiovascular Research·Gemma Chiva-Blanch, Manuel Mayr
Sep 3, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Carmen YapVivian de Waard
Sep 3, 2021·Computational and Structural Biotechnology Journal·Mei-Wei LuanShang-Qian Xie
Oct 8, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Yanming LiYing H Shen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aortic Aneurysm

An aortic aneurysm is the weakening and bulging of the blood vessel wall in the aorta. This causes dilatation of the aorta, which is usually asymptomatic but carries the risk of rupture and hemorrhage. Find the latest research on aortic aneurysms here.

Cardiac Aneurysm

Aneurysm refers to a bulge of the wall or lining of a vessel commonly occurring in the blood vessels at the base of the septum or within the aorta. In the heart, it usually arises from a patch of weakened tissue in a ventricular wall, which swells into a bubble filled with blood. Discover the latest research on cardiac aneurysm here.

Aneurysm

Aneurysms are outward distensions or bulges that occurs in a weakened wall of blood vessels. Discover the latest research on aneurysms here.