Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos

Genome Research
Denghui LiuPing Zheng

Abstract

Naive pluripotency exists in epiblast cells of mouse pre-implantation embryos. However, whether the naive pluripotency is transient or nonexistent in primate embryos remains unclear. Using RNA-seq in single blastomeres from 16-cell embryos through to hatched blastocysts of rhesus monkey, we constructed the lineage segregation roadmap in which the specification of trophectoderm, epiblast, and primitive endoderm is initiated simultaneously at the early blastocyst stage. Importantly, we uncovered the existence of distinct pluripotent states in monkey pre-implantation embryos. At the early- and middle-blastocyst stages, the epiblast cells have the transcriptome features of naive pluripotency, whereas they display a continuum of primed pluripotency characteristics at the late and hatched blastocyst stages. Moreover, we identified potential regulators that might play roles in the transition from naive to primed pluripotency. Thus, our study suggests the transient existence of naive pluripotency in primates and proposes an ideal time window for derivation of primate embryonic stem cells with naive pluripotency.

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Citations

Aug 31, 2019·Biological Research·Bo LvZhigang Xue
Mar 6, 2021·Veterinary Research·Bertrand Pain
Mar 17, 2021·Development·Grace V HancockAmander T Clark
Apr 10, 2021·Proceedings of the National Academy of Sciences of the United States of America·Lixia ZhaoXihe Li
Aug 7, 2021·Nature Reviews. Genetics·Anna D Senft, Todd S Macfarlan
Aug 28, 2021·Nature Communications·Ran YangKenneth R Chien
Oct 4, 2019·Journal of Proteome Research·Sara TaleahmadGhasem Hosseini Salekdeh
Jul 7, 2021·Cell Biochemistry and Function·Reza RanjbarMortaza Taheri-Anganeh

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