Single-cell RT-PCR, in situ hybridization histochemical, and immunohistochemical studies of substance P and enkephalin co-occurrence in striatal projection neurons in rats

Journal of Chemical Neuroanatomy
H B WangAnton Reiner

Abstract

Single-cell RT-PCR studies in 3-4-week-old rats have raised the possibility that as many as 20% of striatal projection neurons may be a unique type that contains both substance P (SP) and enkephalin (ENK). We used single-cell RT-PCR, retrograde labeling, in situ hybridization histochemistry, and immunolabeling to characterize the abundance of this cell type, its projection target(s), and any developmental changes in its frequency. We found by RT-PCR that 11% of neurons containing either SP or ENK contained both in 4-week-old rats, while in 4-month-old rats SP/ENK colocalization was only 3%. SP-only neurons tended to co-contain dynorphin and ENK-only neurons neurotensin, while SP/ENK neurons tended to contain dynorphin. Single-cell RT-PCR showed SP/ENK co-occurrence in 4-week-old rats to be no more common among striatal neurons retrogradely labeled from the substantia nigra than among those retrogradely labeled from globus pallidus. Double-label in situ hybridization showed SP/ENK perikarya to be scattered throughout striatum, making up 8% of neurons containing either SP or ENK at 4 weeks, but only 4% at 4 months. Immunolabeling showed that presumptive striatal terminals in globus pallidus externus, globus pallidus internus and ...Continue Reading

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Citations

Jul 17, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fatuel TecuapetlaMark F Yeckel
Oct 24, 2007·Peptides·Richard J Bodnar
May 29, 2016·Neuroscience and Biobehavioral Reviews·Carina Soares-CunhaAna J Rodrigues
Jul 15, 2016·Brain Structure & Function·Stewart Shipp
Mar 28, 2018·CNS Neuroscience & Therapeutics·Anton Reiner, Yun-Ping Deng
Nov 22, 2007·The European Journal of Neuroscience·Takahiro SonomuraTakeshi Kaneko
Sep 2, 2020·The Journal of Comparative Neurology·Yunping DengAnton Reiner

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