Jan 30, 2020

Single Cell Sequencing and Kidney Organoids Generated from Pluripotent Stem Cells

Clinical Journal of the American Society of Nephrology : CJASN
Haojia Wu, Benjamin D Humphreys


Methods to differentiate human pluripotent stem cells into kidney organoids were first introduced about 5 years ago, and since that time, the field has grown substantially. Protocols are producing increasingly complex three-dimensional structures, have been used to model human kidney disease, and have been adapted for high-throughput screening. Over this same time frame, technologies for massively parallel, single-cell RNA sequencing (scRNA-seq) have matured. Now, both of these powerful approaches are being combined to better understand how kidney organoids can be applied to the understanding of kidney development and disease. There are several reasons why this is a synergistic combination. Kidney organoids are complicated and contain many different cell types of variable maturity. scRNA-seq is an unbiased technology that can comprehensively categorize cell types, making it ideally suited to catalog all cell types present in organoids. These same characteristics also make scRNA-seq a powerful approach for quantitative comparisons between protocols, batches, and pluripotent cell lines as it becomes clear that reproducibility and quality can vary across all three variables. Lineage trajectories can be reconstructed using scRNA-se...Continue Reading

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Mentioned in this Paper

Pluripotent Stem Cells
Kidney Development
Cell Differentiation Process
High-Throughput Screening Assays
Kidney Diseases
Research Activities
Negative Regulation of Cell Differentiation
Cell Type
MRNA Maturation

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