Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart

Cell Reports
Daniel A SkellyAlexander R Pinto


Characterization of the cardiac cellulome, the network of cells that form the heart, is essential for understanding cardiac development and normal organ function and for formulating precise therapeutic strategies to combat heart disease. Recent studies have reshaped our understanding of cardiac cellular composition and highlighted important functional roles for non-myocyte cell types. In this study, we characterized single-cell transcriptional profiles of the murine non-myocyte cardiac cellular landscape using single-cell RNA sequencing (scRNA-seq). Detailed molecular analyses revealed the diversity of the cardiac cellulome and facilitated the development of techniques to isolate understudied cardiac cell populations, such as mural cells and glia. Our analyses also revealed extensive networks of intercellular communication and suggested prevalent sexual dimorphism in gene expression in the heart. This study offers insights into the structure and function of the mammalian cardiac cellulome and provides an important resource that will stimulate studies in cardiac cell biology.

Associated Datasets


Jun 9, 2018·Genes·Michal PawlakCecilia Lanny Winata
Jun 28, 2018·Journal of Leukocyte Biology·Jose Angel Nicolás-ÁvilaIván Ballesteros
Jul 29, 2018·Nature Reviews. Cardiology·Natalie A GudeMark A Sussman
Apr 28, 2018·Circulation Research·Kevin C Wang, Howard Y Chang
Feb 24, 2019·Development·Alexia HulinKatherine E Yutzey
Apr 12, 2019·Current Opinion in Cardiology·Scot J Matkovich
Jan 17, 2019·Annual Review of Immunology·Kevin BasslerJoachim L Schultze
May 23, 2019·Regenerative Medicine·Mark A Sussman
Jun 5, 2019·European Heart Journal·David Rohde, Matthias Nahrendorf
Jul 20, 2019·Journal of the American Heart Association·Yang LiuDeyou Zheng
Aug 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Zhaoning WangEric N Olson
Dec 17, 2019·Science Advances·Micha Sam Brickman RaredonLaura E Niklason
Feb 11, 2020·Annual Review of Physiology·Michelle D Tallquist
Feb 19, 2020·PLoS Computational Biology·Chieh LinZiv Bar-Joseph
May 27, 2020·Circulation·Yang Zhou, Jianyi Zhang
Oct 8, 2019·Cardiovascular Research·Hemant SuryawanshiThomas Tuschl
May 29, 2020·Disease Models & Mechanisms·Margo P CainJichao Chen
May 14, 2020·Bioinformatics·Maria Solovey, Antonio Scialdone
May 15, 2020·Circulation·Nathan R TuckerPatrick T Ellinor
Aug 22, 2020·Circulation·Shu MengJohn P Cooke
Apr 17, 2020·Frontiers in Cardiovascular Medicine·Nicholas W Chavkin, Karen K Hirschi
Jul 8, 2020·International Journal of Molecular Sciences·Olga Bondareva, Bilal N Sheikh
Sep 16, 2020·Experimental & Molecular Medicine·Jean FanFan Zhang
Dec 27, 2018·Journal of Internal Medicine·M Nahrendorf
Mar 1, 2019·Cell Biology and Toxicology·Dongli SongXiangdong Wang
Aug 17, 2019·Frontiers in Cardiovascular Medicine·Mara StellatoGabriela Kania
Mar 13, 2020·APL Bioengineering·Ngan F HuangSong Li

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