Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart

Cell Reports
Daniel A SkellyAlexander R Pinto

Abstract

Characterization of the cardiac cellulome, the network of cells that form the heart, is essential for understanding cardiac development and normal organ function and for formulating precise therapeutic strategies to combat heart disease. Recent studies have reshaped our understanding of cardiac cellular composition and highlighted important functional roles for non-myocyte cell types. In this study, we characterized single-cell transcriptional profiles of the murine non-myocyte cardiac cellular landscape using single-cell RNA sequencing (scRNA-seq). Detailed molecular analyses revealed the diversity of the cardiac cellulome and facilitated the development of techniques to isolate understudied cardiac cell populations, such as mural cells and glia. Our analyses also revealed extensive networks of intercellular communication and suggested prevalent sexual dimorphism in gene expression in the heart. This study offers insights into the structure and function of the mammalian cardiac cellulome and provides an important resource that will stimulate studies in cardiac cell biology.

Associated Datasets

Citations

Jun 9, 2018·Genes·Michal PawlakCecilia Lanny Winata
Jun 28, 2018·Journal of Leukocyte Biology·Jose Angel Nicolás-ÁvilaIván Ballesteros
Jul 29, 2018·Nature Reviews. Cardiology·Natalie A GudeMark A Sussman
Apr 28, 2018·Circulation Research·Kevin C Wang, Howard Y Chang
Feb 24, 2019·Development·Alexia HulinKatherine E Yutzey
Apr 12, 2019·Current Opinion in Cardiology·Scot J Matkovich
Jan 17, 2019·Annual Review of Immunology·Kevin BasslerJoachim L Schultze
May 23, 2019·Regenerative Medicine·Mark A Sussman
Jun 5, 2019·European Heart Journal·David Rohde, Matthias Nahrendorf
Jul 20, 2019·Journal of the American Heart Association·Yang LiuDeyou Zheng
Aug 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Zhaoning WangEric N Olson
Dec 17, 2019·Science Advances·Micha Sam Brickman RaredonLaura E Niklason
Feb 11, 2020·Annual Review of Physiology·Michelle D Tallquist
Feb 19, 2020·PLoS Computational Biology·Chieh LinZiv Bar-Joseph
May 27, 2020·Circulation·Yang Zhou, Jianyi Zhang
Oct 8, 2019·Cardiovascular Research·Hemant SuryawanshiThomas Tuschl
May 29, 2020·Disease Models & Mechanisms·Margo P CainJichao Chen
May 14, 2020·Bioinformatics·Maria Solovey, Antonio Scialdone
May 15, 2020·Circulation·Nathan R TuckerPatrick T Ellinor
Aug 22, 2020·Circulation·Shu MengJohn P Cooke
Apr 17, 2020·Frontiers in Cardiovascular Medicine·Nicholas W Chavkin, Karen K Hirschi
Jul 8, 2020·International Journal of Molecular Sciences·Olga Bondareva, Bilal N Sheikh
Sep 16, 2020·Experimental & Molecular Medicine·Jean FanFan Zhang
Dec 27, 2018·Journal of Internal Medicine·M Nahrendorf
Mar 1, 2019·Cell Biology and Toxicology·Dongli SongXiangdong Wang
Aug 17, 2019·Frontiers in Cardiovascular Medicine·Mara StellatoGabriela Kania
Mar 13, 2020·APL Bioengineering·Ngan F HuangSong Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Plant Journal : for Cell and Molecular Biology
Eric D RogersPhilip N Benfey
Comptes rendus des séances de la Société de biologie et de ses filiales
E SAKIZ
© 2021 Meta ULC. All rights reserved