Single cell transcriptomic profiling of mouse pancreatic progenitors.

Physiological Genomics
Diana E StanescuDoris A Stoffers

Abstract

The heterogeneity of the developing pancreatic epithelium and low abundance of endocrine progenitors limit the information derived from traditional expression studies. To identify genes that characterize early developmental tissues composed of multiple progenitor lineages, we applied single-cell RNA-Seq to embryonic day (e)13.5 mouse pancreata and performed integrative analysis with single cell data from mature pancreas. We identified subpopulations expressing macrophage or endothelial markers and new pancreatic progenitor markers. We also identified potential α-cell precursors expressing glucagon (Gcg) among the e13.5 pancreatic cells. Despite their high Gcg expression levels, these cells shared greater transcriptomic similarity with other e13.5 cells than with adult α-cells, indicating their immaturity. Comparative analysis identified the sodium-dependent neutral amino acid transporter, Slc38a5, as a characteristic gene expressed in α-cell precursors but not mature cells. By immunofluorescence analysis, we observed SLC38A5 expression in pancreatic progenitors, including in a subset of NEUROG3+ endocrine progenitors and MAFB+ cells and in all GCG+ cells. Expression declined in α-cells during late gestation and was absent in th...Continue Reading

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Citations

Apr 9, 2019·Current Protocols in Cell Biology·Rei Sekiguchi, Belinda Hauser
Mar 28, 2019·The EMBO Journal·Zhe Liu, Julie B Sneddon
Oct 28, 2019·Diabetes·E Danielle Dean
Sep 27, 2018·Nature Communications·Lauren E ByrnesJulie B Sneddon
Apr 4, 2021·Pharmaceuticals·Tyler SniegowskiVadivel Ganapathy
May 18, 2021·Frontiers in Cell and Developmental Biology·Wojciech J SzlachcicMalgorzata Borowiak

Methods Mentioned

BETA
nucleic acid amplification
RNA-Seq
chip
scRNA-Seq
PCR
fluorescence-activated cell sorting

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