Single-Cell Transcriptomics Analysis Identifies Nuclear Protein 1 as a Regulator of Docetaxel Resistance in Prostate Cancer Cells.

Molecular Cancer Research : MCR
Patricia M SchneppEvan T Keller

Abstract

The majority of patients with prostate cancer treated with docetaxel develop resistance to it. To better understand the mechanism behind the acquisition of resistance, we conducted single-cell RNA-sequencing (scRNA-seq) of docetaxel-sensitive and -resistant variants of DU145 and PC3 prostate cancer cell lines. Overall, sensitive and resistant cells clustered separately. Differential gene expression analysis between resistant and sensitive cells revealed 182 differentially expressed genes common to both prostate cancer cell lines. A subset of these genes gave a gene expression profile in the resistant transcriptome-like-sensitive cells similar to the resistant cells. Exploration for functional gene pathways identified 218 common pathways between the two cell lines. Protein ubiquitination was the most differentially regulated pathway and was enriched in the resistant cells. Transcriptional regulator analysis identified 321 potential regulators across both cell lines. One of the top regulators identified was nuclear protein 1 (NUPR1). In contrast to the single-cell analysis, bulk analysis of the cells did not reveal NUPR1 as a promising candidate. Knockdown and overexpression of NUPR1 in the prostate cancer cells demonstrated that...Continue Reading

References

Jan 1, 1986·Bulletin of Mathematical Biology·A J Coldman, J H Goldie
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Sep 4, 2003·Genome Research·Paul D ThomasApurva Narechania
Jan 20, 2004·Proceedings. Biological Sciences·Yoh IwasaMartin A Nowak
Mar 9, 2004·The Lancet Oncology·Helen K BerriemanLynn Cawkwell
Apr 8, 2004·Neoplasia : an International Journal for Oncology Research·Daniel R RhodesArul M Chinnaiyan
Oct 8, 2004·The New England Journal of Medicine·Ian F TannockUNKNOWN TAX 327 Investigators
Oct 8, 2004·The New England Journal of Medicine·Daniel P PetrylakE David Crawford
Feb 2, 2008·The Lancet Oncology·Pascal Sève, Charles Dumontet
Jan 21, 2009·Cancer Metastasis Reviews·Uttio Roy ChowdhuryLalita A Shevde
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Sep 22, 2012·The Prostate·Chengfei LiuAllen C Gao
Sep 26, 2013·Nature Reviews. Cancer·Caitriona HolohanPatrick G Johnston
Dec 18, 2013·Bioinformatics·Andreas KrämerStuart Tugendreich
Apr 1, 2014·Journal of Theoretical Biology·Jasmine Foo, Franziska Michor
May 20, 2014·Nature Methods·Peter V KharchenkoDavid T Scadden
Sep 4, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jiangxin WangWeiwen Zhang
Apr 16, 2016·Asian Journal of Andrology·Jonathan Shoag, Christopher E Barbieri
May 23, 2017·Therapeutic Advances in Medical Oncology·Javier PuenteJose Angel Arranz
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
May 22, 2019·Nature Immunology·Evan DerUNKNOWN Accelerating Medicines Partnership Rheumatoid Arthritis and Systemic Lupus Erythematosus (AMP RA/SLE) Consortium

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