Single-Cell Transcriptomics of Human Oocytes: Environment-Driven Metabolic Competition and Compensatory Mechanisms During Oocyte Maturation

Antioxidants & Redox Signaling
Hongcui ZhaoJie Qiao

Abstract

The mechanisms coordinating maturation with an environment-driven metabolic shift, a critical step in determining the developmental potential of human in vitro maturation (IVM) oocytes, remain to be elucidated. Here we explored the key genes regulating human oocyte maturation using single-cell RNA sequencing and illuminated the compensatory mechanism from a metabolic perspective by analyzing gene expression. Three key genes that encode CoA-related enzymes were screened from the RNA sequencing data. Two of them, ACAT1 and HADHA, were closely related to the regulation of substrate production in the Krebs cycle. Dysfunction of the Krebs cycle was induced by decreases in the activity of specific enzymes. Furthermore, the activator of these enzymes, the calcium concentration, was also decreased because of the failure of influx of exogenous calcium. Although release of endogenous calcium from the endoplasmic reticulum and mitochondria met the requirement for maturation, excessive release resulted in aneuploidy and developmental incompetence. High nicotinamide nucleotide transhydrogenase expression induced NADPH dehydrogenation to compensate for the NADH shortage resulting from the dysfunction of the Krebs cycle. Importantly, high NAD...Continue Reading

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Oct 20, 2018·The Analyst·Lei YinJingkai Gu
Apr 15, 2020·Zygote : the Biology of Gametes and Early Embryos·Sofia CoelhoMário Sousa
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Datasets Mentioned

BETA
GSE110798

Methods Mentioned

BETA
dot blot
PCR
Illumina sequencing
DNA Assay
ELISA
ELISAs

Software Mentioned

ENSEMBL
NIH ImageJ
eXpress
Cluster
Java
DAVID
BlueGnome
DESeq
Curve Expert
TopHat

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