Single-chain Fv antibody with specificity for Listeria monocytogenes

Journal of Immunological Methods
George C PaoliJeffrey D Brewster

Abstract

Single chain antibodies (scFv) exhibiting specific binding to Listeria monocytogenes strains were isolated from a pool of random scFvs expressed on the surface of filamentous bacteriophages. Positive selection (panning) using L. monocytogenes was used to enrich for phage clones with the desired binding affinity, and negative selection using L. innocua and L. ivanovii was used to remove phages expressing cross-reactive antibody fragments. A single phage clone, P4:A8, was selected using two independent panning schemes. A rapid assay was devised to determine phage antibody binding specificity and was used to develop a selectivity profile for individual phage clones. The P4:A8 clone was screened against a panel of bacteria consisting of eight strains of L. monocytogenes, one each of the other six species of Listeria and nine other relevant bacterial species. A collection of individual clones from the penultimate panning was also screened against a subset of the panel of bacteria. The selectivity profiles indicate that multiple clones, including P4:A8, exhibit binding to one or more strains of L. monocytogenes without cross-reactivity toward any other species in the panel. This is the first report of a species-specific antibody for ...Continue Reading

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Citations

Feb 20, 2008·Analytical and Bioanalytical Chemistry·Ilaria Palchetti, Marco Mascini
Mar 24, 2007·Foodborne Pathogens and Disease·George C PaoliJeffrey D Brewster
Jun 6, 2012·Antimicrobial Agents and Chemotherapy·Johnny X HuangMatthew A Cooper
Jun 22, 2011·Annual Review of Analytical Chemistry·Andrew G Gehring, Shu-I Tu
May 22, 2007·Biosensors & Bioelectronics·Viswaprakash NanduriJeffrey D Brewster
Nov 29, 2005·Journal of Microbiological Methods·Robin L T ChurchillJ Christopher Hall
May 10, 2017·Journal of Immunology Research·Elena CriscuoloRoberto Burioni
Apr 27, 2021·Critical Reviews in Microbiology·Leonardo Lopes-LuzSamira Bührer-Sékula

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