Single chain-urokinase binds to oxidized fibrin(ogen) derivatives

Thrombosis Research
T W StiefJ M Rodriguez

Abstract

Single chain- urokinase (scu-PA), in contrast to two chain urokinase, is a physiological plasminogen activator of fibrin selectivity. The mechanism of selective fibrinolysis of scu- PA has not been clarified up to now. This study has shown that fibrin selectivity might involve oxidative processes. Binding studies with immobilized oxidized fibrinogen degradation products (FDP) demonstrated higher affinity of scu-PA to oxidized than to unmodified FDP. The fibrin(ogen) domain responsible for this oxidant mediated increase of scu-PA affinity is localized in the D-subunit of fibrin(ogen). Thus, experimental data upon the interaction of scu-PA with fibrin(ogen) using oxidized and I- labelled fibrin(ogen) might be interpreted with caution: the oxidized product may behave in a distinct manner than the unoxidized, native, one. Activated leukocytes release large amounts of scu-PA and of oxidants of the chloramine type. The oxidants could contribute significantly to fibrinolysis and proteolysis in areas of inflammation, preparing fibrin for its specific degradation. The present data support the concept of an involvement of oxidative processes in the fibrinolytic pathway.

References

Sep 1, 1975·Archives of Biochemistry and Biophysics·B N ViolandF J Castellino
Oct 7, 1975·Biochemistry·Y ShechterA Patchornik
Feb 9, 1989·The New England Journal of Medicine·S J Weiss
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S D WrightJ D Loike
Oct 21, 1986·Biochemistry·D A Lawrence, D J Loskutoff
Apr 1, 1987·Seminars in Thrombosis and Hemostasis·V Gurewich, R Pannell
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S R Gonda, J R Shainoff

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