The single-channel behavior of the hyperpolarization-activated, ClC-2-like inwardly rectifying Cl- current (IClh), induced by long-term dibutyryl-cyclic-AMP-treated cultured cortical rat astrocytes, was analyzed with the patch-clamp technique. In outside-out patches in symmetrical 144 mM Cl-solutions, openings of hyperpolarization-activated small-conductance Cl channels revealed burst activity of two equidistant conductance levels of 3 and 6 pS. The unitary openings displayed slow activation kinetics. The probabilities of the closed and conducting states were consistent with a double-barrelled structure of the channel protein. These results suggest that the astrocytic ClC-2-like Cl- current Iclh is mediated by a small-conductance Cl channel, which has the same structural motif as the Cl- channel prototype CIC-0.
Single-channel characteristics of the large-conductance anion channel in rat cortical astrocytes in primary culture
Two distinct inwardly rectifying conductances are expressed in long term dibutyryl-cyclic-AMP treated rat cultured cortical astrocytes
Activation of hyperpolarization and atypical osmosensitivity of a Cl- current in rat osteoblastic cells
Modulation of the hyperpolarization-activated Cl- current in human intestinal T84 epithelial cells by phosphorylation
Characterization of an inwardly rectifying chloride conductance expressed by cultured rat cortical astrocytes
Characterization of the hyperpolarization-activated chloride current in dissociated rat sympathetic neurons
The muscle chloride channel ClC-1 has a double-barreled appearance that is differentially affected in dominant and recessive myotonia
Functional characterization of a ClC-2-like Cl(-) conductance in surface epithelial cells of rat rectal colon.
Functional evaluation of human ClC-2 chloride channel mutations associated with idiopathic generalized epilepsies
Chlorotoxin-sensitive Ca2+-activated Cl- channel in type R2 reactive astrocytes from adult rat brain
Osmosensitivity of an inwardly rectifying chloride current revealed by whole-cell and perforated-patch recordings in cultured rat cortical astrocytes
Functional characterization of novel alternatively spliced ClC-2 chloride channel variants in the heart
Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.