Single-channel analysis of a ClC-2-like chloride conductance in cultured rat cortical astrocytes

FEBS Letters
M NobileS Ferroni

Abstract

The single-channel behavior of the hyperpolarization-activated, ClC-2-like inwardly rectifying Cl- current (IClh), induced by long-term dibutyryl-cyclic-AMP-treated cultured cortical rat astrocytes, was analyzed with the patch-clamp technique. In outside-out patches in symmetrical 144 mM Cl-solutions, openings of hyperpolarization-activated small-conductance Cl channels revealed burst activity of two equidistant conductance levels of 3 and 6 pS. The unitary openings displayed slow activation kinetics. The probabilities of the closed and conducting states were consistent with a double-barrelled structure of the channel protein. These results suggest that the astrocytic ClC-2-like Cl- current Iclh is mediated by a small-conductance Cl channel, which has the same structural motif as the Cl- channel prototype CIC-0.

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C K BauerT J Jentsch
Aug 22, 1986·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P T Gray, J M Ritchie
Dec 1, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C Miller
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C Miller, M M White
Jan 1, 1995·The Journal of Physiology·T J JentschB Schwappach
Jun 1, 1994·The Journal of Membrane Biology·D Chesnoy-Marchais, J Fritsch
Jan 15, 1996·The Journal of Physiology·J ArreolaT Begenisich
Sep 26, 1996·Nature·R E MiddletonC Miller
Jan 1, 1997·The Journal of General Physiology·M PuschT J Jentsch
Aug 1, 1997·Biophysical Journal·U LudewigT J Jentsch
Aug 26, 1998·Nature·C FahlkeA L George
Jun 17, 1999·Pflügers Archiv : European journal of physiology·T J JentschH Yamada

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Citations

Mar 22, 2006·The Journal of Membrane Biology·G UgarteJ Bacigalupo
Apr 23, 2010·The Journal of Membrane Biology·Akihiro InagakiToru Ishikawa
Feb 16, 2005·Annual Review of Physiology·Thomas J JentschAnselm A Zdebik
May 31, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hui Fang BaoDouglas C Eaton
Aug 17, 2001·The Journal of Physiology·G L PetheoA Spät
Aug 23, 2001·Current Biology : CB·A L GeorgeC G Vanoye
Oct 24, 2014·Frontiers in Physiology·Gabriel StöltingChristoph Fahlke
Sep 19, 2002·Glia·Wolfgang Walz
May 31, 2018·Physiological Reviews·Thomas J Jentsch, Michael Pusch
Mar 28, 2002·Physiological Reviews·Thomas J JentschAnselm A Zdebik
Mar 11, 2006·The Biochemical Journal·Mohabir RamjeesinghChristine E Bear
May 15, 2001·American Journal of Physiology. Cell Physiology·M BaliJ Fritsch
May 24, 2017·Molecular Medicine Reports·Hongwei WangXin Wang
Dec 20, 2018·American Journal of Physiology. Cell Physiology·Viktor YarotskyyGeorgi V Petkov
May 11, 2005·The Journal of Biological Chemistry·Fiona C BrittonDayue Duan

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