PMID: 8843728Sep 1, 1996Paper

Single-channel basis of slow inactivation of Na+ channels in rat skeletal muscle

The American Journal of Physiology
R L Ruff

Abstract

This study examined the single-channel basis of slow inactivation of Na+ currents (INa) in rat fast-twitch skeletal muscle fibers. A loose patch voltage clamp monitored changes in the maximum inward INa as the holding potential of the membrane patch changed. On a neighboring region of extrajunctional membrane of the same fiber, a gigaohm seal patch voltage clamp recorded single-channel INa. The maximum number of simultaneously open Na+ channels among a group of current traces indicated the maximum number of excitable channels. The holding potentials of the two voltage clamps were the same. Slow inactivation did not affect the open time or conductance of single Na+ channels. The number of excitable Na+ channels reversibly decreased during development of slow inactivation of INa and increased during recovery from slow inactivation of INa. Different stimulation protocols examined whether Na+ channels had to be in the closed, open, or fast-inactivated states to enter the slow-inactivated state. Na+ channels appear to be able to enter the slow-inactivated state from the closed, open, or fast-inactivated state.

References

May 1, 1977·The Journal of Physiology·H Meves, W Vogel
Oct 1, 1992·Physiological Reviews·W A Catterall
Sep 1, 1992·The Journal of Physiology·J R Howe, J M Ritchie
Oct 1, 1991·Physiological Reviews·J Patlak
Nov 1, 1991·The Journal of Physiology·J H LawrenceE Marban
Jan 1, 1990·Pflügers Archiv : European journal of physiology·C Franke, H Hatt
Mar 1, 1990·The Journal of General Physiology·B E ScanleyH A Fozzard
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J M BekkersN G Greeff
Feb 1, 1987·The Journal of Physiology·R L RuffW Stühmer
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P VassilevW A Catterall
Oct 1, 1984·The Journal of General Physiology·C A Vandenberg, R Horn
Jul 1, 1981·Physiological Reviews·C M Armstrong
Jan 1, 1982·The Journal of General Physiology·F BezanillaJ M Fernández
Aug 1, 1991·Biophysical Journal·R Horn

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Citations

Jul 1, 1997·The Journal of General Physiology·C Townsend, R Horn
Jan 16, 2008·Physiological Reviews·D G AllenH Westerblad
Sep 13, 2008·Clinical and Experimental Pharmacology & Physiology·Chris van der PoelD George Stephenson
Jan 20, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K T Murphy, T Clausen
Jun 9, 1998·Annals of the New York Academy of Sciences·R L Ruff
Sep 27, 2005·Physiological Reviews·Werner Ulbricht
Nov 3, 2006·The Journal of Clinical Investigation·Bianca M Conti-FineHenry J Kaminski
Apr 27, 2007·American Journal of Physiology. Cell Physiology·Joshua N EdwardsD George Stephenson
Jul 18, 2001·Annals of Neurology·M M Rich, M J Pinter

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