Single-channel currents produced by the serotonin transporter and analysis of a mutation affecting ion permeation

Biophysical Journal
F LinS Mager

Abstract

Single-channel activities were observed in outside-out patches excised from oocytes expressing a mammalian 5-hydroxytryptamine (5-HT) transporter. Channel conductance was larger for a mutant in which asparagine177 of the third putative transmembrane domain was replaced by glycine, suggesting that this residue lies within or near the permeation pathway. The N177G mutant enables quantitative single-channel measurements; it displays two conducting states. One state, with conductance of approximately 6 pS, is induced by 5-HT and is permeable to Na+. The other state (conductance of approximately 13 pS) is associated with substrate-independent leakage current and is permeable to both Na+ and Li+. Cl- is not a major current carrier. Channel lifetimes under all conditions measured are approximately 2.5 ms. The single-channel phenomena account for previously observed macroscopic electrophysiological phenomena, including 5-HT-induced transport-associated currents and substrate-independent leakage currents. The channel openings occur several orders of magnitude less frequently than would be expected if one such opening occurred for each transport cycle and therefore do not represent an obligatory step in transport. Nevertheless, single-ch...Continue Reading

References

Oct 25, 1991·Science·B J HoffmanM J Brownstein
Sep 14, 1990·Science·J GuastellaB I Kanner
Jan 1, 1990·Annals of the New York Academy of Sciences·R W Fuller, D T Wong
Sep 1, 1995·Neuron·J I WadicheM P Kavanaugh
Jan 1, 1994·Annual Review of Pharmacology and Toxicology·H A LesterJ L Corey
Jan 1, 1993·Annual Review of Neuroscience·S G Amara, M J Kuhar
Oct 4, 1993·Biochimica Et Biophysica Acta·G Rudnick, J Clark
Apr 1, 1994·Neuron·S MagerH A Lester
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·J N Cammack, E A Schwartz
Feb 1, 1996·Biophysical Journal·L J DeFelice, R D Blakely
Feb 1, 1996·Biophysical Journal·H P LarssonH Lecar

❮ Previous
Next ❯

Citations

Jun 29, 2005·The Journal of Membrane Biology·M H KarakossianS Eskandari
Apr 10, 2007·The Journal of Membrane Biology·Gary Rudnick
May 11, 2011·Pflügers Archiv : European journal of physiology·Alberto RivettaClifford Slayman
Aug 26, 1998·Drug and Alcohol Dependence·S G Amara, M S Sonders
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lucia CarvelliLouis J Defelice
Sep 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lucia CarvelliLouis J DeFelice
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·M P Kavanaugh
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·A GalliL J DeFelice
Apr 14, 2009·The Journal of Biological Chemistry·Anne-Kristine MeinildNanna MacAulay
Nov 7, 2009·The Journal of Biological Chemistry·Jacob AndersenAnders S Kristensen
Nov 11, 2011·The Journal of Biological Chemistry·Klaus SchickerWalter Sandtner
Mar 28, 2012·The Journal of Biological Chemistry·Simon BullingWalter Sandtner
Mar 7, 1998·The Journal of General Physiology·H A Lester, D A Dougherty
Oct 25, 2006·Annual Review of Physiology·Louis J DeFelice, Tapasree Goswami
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kristopher M KahligAurelio Galli
Jul 23, 2009·Neurochemistry International·Edward MatthewsSepehr Eskandari
Nov 21, 2013·British Journal of Pharmacology·Walter SandtnerHarald H Sitte
Jun 25, 2002·Biophysical Journal·Ming Li, Henry A Lester
Oct 29, 2013·Neurochemistry International·Matthew L BeckmanL Keith Henry
Dec 24, 2002·Biophysical Journal·Scott V Adams, Louis J DeFelice
Feb 14, 2018·The Journal of General Physiology·Peter S HasenhuetlWalter Sandtner
Jun 25, 2004·The Journal of Membrane Biology·T KurodaA Rivetta
Aug 20, 2016·F1000Research·Louis J De Felice
Jul 19, 2005·Physiology·Randy D BlakelyAurelio Galli
Jul 27, 2014·The Journal of Biological Chemistry·Lars BorreUlrik Gether
Oct 2, 2019·The Journal of General Physiology·Gary Rudnick, Walter Sandtner
Oct 31, 2019·International Journal of Molecular Sciences·Verena BurtscherWalter Sandtner
Nov 27, 1999·The Journal of Biological Chemistry·X Z ChenM A Hediger

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Next ❯

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