PMID: 7544823May 1, 1995Paper

Single-channel properties of a volume-sensitive anion conductance. Current activation occurs by abrupt switching of closed channels to an open state

The Journal of General Physiology
P S Jackson, K Strange

Abstract

Swelling-induced loss of organic osmolytes from cells is mediated by an outwardly rectified, volume-sensitive anion channel termed VSOAC (Volume-Sensitive Organic osmolyte/Anion Channel). Similar swelling-activated anion channels have been described in numerous cell types. The unitary conductance and gating kinetics of VSOAC have been uncertain, however. Stationary noise analysis and single-channel measurements have produced estimates for the unitary conductance of swelling-activated, outwardly rectified anion channels that vary by > 15-fold. We used a combination of stationary and nonstationary noise analyses and single-channel measurements to estimate the unitary properties of VSOAC. Current noise was analyzed initially by assuming that graded changes in macroscopic current were due to graded changes in channel open probability. Stationary noise analysis predicts that the unitary conductance of VSOAC is approximately 1 pS at 0 mV. In sharp contrast, nonstationary noise analysis demonstrates that VSOAC is a 40-50 pS channel at +120 mV (approximately 15 pS at 0 mV). Measurement of single-channel events in whole-cell currents and outside-out membrane patches confirmed the nonstationary noise analysis results. The discrepancy bet...Continue Reading

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Citations

Sep 1, 1996·Metabolic Brain Disease·H Pasantes-Morales
Oct 1, 1996·Molecular Neurobiology·S Basavappa, J C Ellory
Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Mar 13, 1998·Journal of Neuroscience Methods·R Steffan, S H Heinemann
Oct 1, 1996·General Pharmacology·B NiliusG Droogmans
Jun 1, 1996·Current Opinion in Neurobiology·T J Jentsch
May 1, 1995·The Journal of General Physiology·P S Jackson, K Strange
Sep 1, 1997·The Journal of General Physiology·T VoetsB Nilius
Jul 2, 2003·The Journal of General Physiology·Konstantin GusevGalina N Mozhayeva
Dec 31, 2003·The Journal of General Physiology·Victor G RomanenkoIrena Levitan
Jan 28, 2004·The Journal of General Physiology·Andriy V YerominMichael D Cahalan
Aug 31, 2006·The Journal of General Physiology·Murali Prakriya, Richard S Lewis
Apr 1, 1997·The EMBO Journal·S E Jordt, T J Jentsch
Mar 11, 2004·Clinical and Experimental Pharmacology & Physiology·Steve J CullifordRoland Z Kozlowski
Apr 29, 1998·Annual Review of Physiology·K Kirk, K Strange
Feb 5, 2002·Annual Review of Physiology·Xinhua Li, Steven A Weinman
Jan 25, 2012·International Journal of Molecular Sciences·Ranokhon S KurbannazarovaRavshan Z Sabirov
May 16, 1998·The Journal of Cell Biology·A Lepple-WienhuesF Lang
Jan 8, 2016·Pflügers Archiv : European journal of physiology·Stine F PedersenBernd Nilius
Jan 6, 2004·Fundamental & Clinical Pharmacology·Alexandra d'Anglemont de TassignyAlain Berdeaux
Aug 16, 2016·Pflügers Archiv : European journal of physiology·Roberta BenedettoKarl Kunzelmann
Oct 23, 2004·FEBS Letters·Vadim I TernovskyRavshan Z Sabirov
Jun 16, 2000·The Journal of Physiology·A L Pérez-SamartínR O Arellano
Oct 12, 2007·The European Journal of Neuroscience·Guillaume DucharmeLyanne C Schlichter
Mar 28, 2002·Physiological Reviews·Thomas J JentschAnselm A Zdebik
Jan 18, 2019·The Journal of General Physiology·Kevin StrangeJerod S Denton
Sep 9, 1998·The American Journal of Physiology·G DroogmansB Nilius
May 24, 2017·Molecular Medicine Reports·Hongwei WangXin Wang
Dec 31, 1997·The American Journal of Physiology·A S MonaghanF V Sepúlveda
Jan 5, 2000·Physiological Reviews·J R HumeB Horowitz
Oct 16, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Gong Xin LiuWilliam A Coetzee

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