Single channel recording of a mitochondrial calcium uniporter

Biochemical and Biophysical Research Communications
Guangyan WuYuequan Shen

Abstract

Mitochondrial calcium uniporter (MCU) is the pore-forming subunit of the entire uniporter complex and plays an important role in mitochondrial calcium uptake. However, the single channel recording of MCU remains controversial. Here, we expressed and purified different MCU proteins and then reconstituted them into planar lipid bilayers for single channel recording. We showed that MCU alone from Pyronema omphalodes (pMCU) is active with prominent single channel Ca2+currents. In sharp contrast, MCU alone from Homo sapiens (hMCU) is inactive. The essential MCU regulator (EMRE) activates hMCU, and therefore, the complex (hMCU-hEMRE) shows prominent single channel Ca2+currents. These single channel currents are sensitive to the specific MCU inhibitor Ruthenium Red. Our results clearly demonstrate that active MCU can conduct large amounts of calcium into the mitochondria.

Citations

Nov 16, 2018·British Journal of Pharmacology·Luigi LeanzaIldiko Szabo
Apr 23, 2019·PLoS Biology·Xiaofen LiuYuequan Shen
Jun 13, 2018·The Journal of General Physiology·Chen-Wei Tsai, Ming-Feng Tsai
Oct 2, 2019·International Journal of Molecular Sciences·Nadezhda V TarasovaAndrey V Elchaninov
Jun 30, 2018·Science·Jiho YooSeok-Yong Lee
Dec 3, 2020·Cell Calcium·Simona FenoDenis Vecellio Reane
May 1, 2020·Journal of Molecular and Cellular Cardiology·Joanne F GarbinciusJohn W Elrod
Jan 27, 2019·Archives of Biochemistry and Biophysics·Jessica L CaoJin O-Uchi
Oct 27, 2021·Physiological Reviews·Joanne F Garbincius, John W Elrod

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