DOI: 10.1101/500892Dec 19, 2018Paper

Single-copy Knock-In Loci for Defined Gene Expression in C. elegans

BioRxiv : the Preprint Server for Biology
Carlos G Silva-GarciaWilliam B Mair

Abstract

We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any cDNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any cDNA sequence can be introduced as a single integrated copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical and effective approach for generating single-copy non-silenced transgenes in C. elegans .

Related Concepts

Biological Markers
DNA, Complementary
Gene Expression
Genome
Oligonucleotides, Antisense
Promoter
Caenorhabditis elegans
Transgenes
BAT Loci
Regulation of Biological Process

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