Single-crossover integration in the Lactobacillus sake chromosome and insertional inactivation of the ptsI and lacL genes.

Applied and Environmental Microbiology
L LeloupF Morel-Deville

Abstract

Single-crossover homologous integration in Lactobacillus sake was studied. Integration was conducted with nonreplicative delivery vector pRV300. This vector is composed of a pBluescript SK- replicon for propagation in Escherichia coli and an erythromycin resistance marker. Random chromosomal DNA fragments of L. sake 23K ranging between 0.3 and 3.4 kb were inserted into pRV300. The resulting plasmids were able to integrate into the chromosome by homologous recombination as single copies and were maintained stably. The single cross-over integration frequency was logarithmically proportional to the extent of homology between 0.3 and 1.2 kb and reached a maximum value of 1.4 x 10(3) integrants/micrograms of DNA. We used this integration strategy to inactivate the ptsI gene, encoding enzyme I of the phosphoenolpyruvate:carbohydrate phosphotransferase system, and the lacL gene, which is one of the two genes required for the synthesis of a functional beta-galactosidase. The results indicated that our method facilitates genetic analysis of L. sake.

References

Sep 1, 1991·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Feb 1, 1989·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Jul 1, 1989·Applied and Environmental Microbiology·M C ChopinN Galleron
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Sep 1, 1988·Journal of Bacteriology·V Vagner, S D Ehrlich
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V M WattW J Rutter
Sep 1, 1983·Applied and Environmental Microbiology·D G Anderson, L L McKay
Dec 1, 1995·Microbiology·M ObstW P Hammes
Jun 1, 1997·Applied and Environmental Microbiology·R StentzM Zagorec
Sep 1, 1990·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Jun 1, 1996·Applied and Environmental Microbiology·R LauretM Zagorec
Jan 1, 1994·Meat Science·W P Hammes, H J Knauf
May 1, 1996·Microbiology·Françoise BerthierFrançoise Morel-Deville

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Citations

Jan 5, 2005·Journal of Bacteriology·Karin VidoPhilippe Gaudu
Dec 3, 2004·Applied and Environmental Microbiology·Anika MarceauMarie-Christine Champomier-Vergès
Aug 19, 2007·Journal of Molecular Microbiology and Biotechnology·Sophie BeaufilsAxel Hartke
Jul 18, 2009·Journal of Industrial Microbiology & Biotechnology·Inmaculada NadalVicente Monedero
Dec 25, 2010·Applied and Environmental Microbiology·Cristina AlcántaraManuel Zúñiga
May 1, 2012·Applied and Environmental Microbiology·Jesús Rodríguez-DíazMaría J Yebra
Jul 31, 2012·Applied and Environmental Microbiology·E MorelloP Langella
Jan 22, 2013·Applied and Environmental Microbiology·Jamila Anba-MondoloniMarie-Christine Champomier-Vergès
Mar 5, 2013·Applied and Environmental Microbiology·Ainhoa Revilla-GuarinosManuel Zúñiga
Jul 15, 2015·Applied and Environmental Microbiology·Bokyung LeeMaria L Marco
Mar 30, 2016·The Journal of Biological Chemistry·Ana SolopovaSaulius Kulakauskas
Jul 19, 2020·Applied and Environmental Microbiology·Emilie VerplaetseMarie-Christine Champomier-Vergès
Oct 9, 2002·Molecular Microbiology·Carine MercierSaulius Kulakauskas
Sep 6, 2003·Applied and Environmental Microbiology·Carl-Alfred AlpertMonique Zagorec
Oct 31, 2002·Applied and Environmental Microbiology·Linda D RhodesMark S Strom
May 9, 2007·The Journal of Biological Chemistry·Patrick VeigaSaulius Kulakauskas
Apr 24, 2007·Applied and Environmental Microbiology·María Jesús YebraVicente Monedero
Nov 5, 2005·Applied and Environmental Microbiology·Christophe GittonMichel-Yves Mistou
Aug 23, 2011·Biotechnology Journal·Clemens PeterbauerDietmar Haltrich
Jan 31, 2008·Journal of Industrial Microbiology & Biotechnology·Juan RicoVicente Monedero
Mar 24, 2012·PloS One·Nicolas TrémillonIsabelle Poquet
Sep 9, 2009·Journal of Applied Microbiology·D Muñoz-ProvencioV Monedero
Jun 5, 2019·Molecular Biotechnology·Narisetty VivekParameswaran Binod
Mar 8, 2002·Microbiology·María J GosalbesGaspar Pérez-Martínez
Aug 30, 2002·Applied and Environmental Microbiology·Patrice GaurivaudPatrícia B Monteiro

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