Single-cycle kinetic analysis of ternary DNA complexes by surface plasmon resonance on a decaying surface

Biochimie
William Palau, Carmelo Di Primo

Abstract

Complexes involving three DNA strands were used to demonstrate that the single-cycle kinetics (SCK) method, which consists in injecting sequentially samples at increasing concentrations and until now used exclusively to investigate bimolecular complexes by surface plasmon resonance, can be extended to the kinetic analysis of ternary complexes. DNA targets, B, were designed with sequences of variable lengths on their 3' sides that recognise a surface-immobilized biotinylated DNA anchor, A. These targets displayed on their 5' sides sequences that recognise DNA oligonucleotides of variable lengths, C, namely the analytes. Combinations of B and C DNA oligonucleotides on A generated ternary complexes each composed of two Watson-Crick helices displaying different kinetic properties. The target-analyte B-C duplexes were formed by sequentially injecting three increasing concentrations of the analytes C during the dissociation phase of the target B from the anchor A. The sensorgrams for the target-analyte complexes dissociating from the functionalized surface were successfully fitted by the SCK method while the target dissociated from the anchor, i.e. on a decaying surface. Within the range of applicability of the method which is driven...Continue Reading

References

Aug 1, 1980·Medical Hypotheses·S Cantor
Nov 11, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bryan BernatAnthony A Kossiakoff
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Scott T R WalshAnthony A Kossiakoff
Dec 13, 2005·Analytical Biochemistry·Robert KarlssonDavid G Myszka
Sep 6, 2007·The Journal of Biological Chemistry·Jean-Baptiste JomainVincent Goffin
Aug 13, 2009·Chembiochem : a European Journal of Chemical Biology·Céline Douat-CasassusStéphane Quideau
Jun 7, 2011·Journal of Nanobiotechnology·Eric DausseJean-Jacques Toulmé
Nov 1, 2011·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka

❮ Previous
Next ❯

Citations

Feb 8, 2014·ACS Nano·Esha SenguptaDavid S Ginger
Jul 15, 2015·Journal of Biochemistry·Masato HamasakiMakoto Kimura
Oct 10, 2013·Chembiochem : a European Journal of Chemical Biology·Mario A E RebhanJonathan Hall
Jul 9, 2016·Nature Communications·Antonio Luis Egea-JimenezPascale Zimmermann
Jan 22, 2019·Nature Communications·Nicola FendericoMadelon M Maurice
Oct 28, 2021·Microbiology Spectrum·Christine E Broster ReixDerrick R Robinson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.