Single-dose intranasal administration of AdCOVID elicits systemic and mucosal immunity against SARS-CoV-2 in mice.

BioRxiv : the Preprint Server for Biology
Rodney G KingScot Roberts

Abstract

The coronavirus disease 2019 (COVID-19) pandemic has highlighted the urgent need for effective preventive vaccination to reduce burden and spread of severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) in humans. Intranasal vaccination is an attractive strategy to prevent COVID-19 as the nasal mucosa represents the first-line barrier to SARS-CoV-2 entry before viral spread to the lung. Although SARS-CoV-2 vaccine development is rapidly progressing, the current intramuscular vaccines are designed to elicit systemic immunity without conferring mucosal immunity. Here, we show that AdCOVID, an intranasal adenovirus type 5 (Ad5)-vectored vaccine encoding the receptor binding domain (RBD) of the SARS-CoV-2 spike protein, elicits a strong and focused immune response against RBD through the induction of mucosal IgA, serum neutralizing antibodies and CD4+ and CD8+ T cells with a Th1-like cytokine expression profile. Therefore, AdCOVID, which promotes concomitant systemic and local mucosal immunity, represents a promising COVID-19 vaccine candidate.

Citations

Feb 3, 2021·The Journal of Clinical Investigation·Kenta MatsudaMark Connors
Mar 2, 2021·Journal of Drug Delivery Science and Technology·Ahmed A H AbdellatifHelal F Hetta
Feb 24, 2021·NPJ Vaccines·Nikolaos C KyriakidisEsteban Ortiz Prado
Mar 7, 2021·Viruses·Kenneth Lundstrom
Apr 19, 2021·Current Infectious Disease Reports·Arun KumarPaul A Kristiansen
May 9, 2021·International Journal of Pharmaceutics·Mattia TiboniLisbeth Illum

Datasets Mentioned

BETA
QHD43416

Methods Mentioned

BETA
bronchoalveolar
flow cytometry
lavage

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