Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2

BioRxiv : the Preprint Server for Biology
Xingyue AnNavin Varadarajan


A safe and durable vaccine is urgently needed to tackle the COVID19 pandemic that has infected >15 million people and caused >620,000 deaths worldwide. As with other respiratory pathogens, the nasal compartment is the first barrier that needs to be breached by the SARS-CoV-2 virus before dissemination to the lung. Despite progress at remarkable speed, current intramuscular vaccines are designed to elicit systemic immunity without conferring mucosal immunity. We report the development of an intranasal subunit vaccine that contains the trimeric or monomeric spike protein and liposomal STING agonist as adjuvant. This vaccine induces systemic neutralizing antibodies, mucosal IgA responses in the lung and nasal compartments, and T-cell responses in the lung of mice. Single-cell RNA-sequencing confirmed the concomitant activation of T and B cell responses in a germinal center-like manner within the nasal-associated lymphoid tissues (NALT), confirming its role as an inductive site that can lead to long-lasting immunity. The ability to elicit immunity in the respiratory tract has can prevent the initial establishment of infection in individuals and prevent disease transmission across humans.


Dec 17, 2020·ACS Biomaterials Science & Engineering·Benjamin CossetteJoel H Collier
May 9, 2021·International Journal of Pharmaceutics·Mattia TiboniLisbeth Illum

Methods Mentioned

dynamic light scattering
electrophoretic light scattering
bronchoalveolar lavage
Fluorescence-Activated Cell Sorting

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

BioRxiv : the Preprint Server for Biology
Navin Varadaraj AnNavin Varadarajan
Drug Delivery and Translational Research
Mehfuz ZamanIstvan Toth
The American Journal of Tropical Medicine and Hygiene
Prosper N BoyakaJ R McGhee
© 2021 Meta ULC. All rights reserved