Single-drop microextraction and gas chromatography/mass spectrometric determination of anisaldehyde isomers in human urine and blood serum

Rapid Communications in Mass Spectrometry : RCM
Bo-Min LiuHui-Fen Wu

Abstract

The application of single-drop microextraction (SDME) followed by gas chromatography/chemical ionization mass spectrometry (GC/CI-MS) was investigated for the determination of anisaldehyde isomers in human urine and blood serum. The effects of extraction solvent, sample agitation rate, salt addition, sampling time and temperature on the extraction efficiency were examined and optimized. Analytical parameters such as linearity, reproducibility, detection limit and relative recovery were evaluated under the optimized experimental conditions. Good reproducibilities of replicate extractions (n = 5) were obtained, with relative standard deviation (RSD) values below 6%. The limits of detection (LOD) using an extraction time of 5 min were found to be in the range 2-5 ng/mL under the selected ion monitoring (SIM) mode of GC/MS. Recoveries of 82-98% were achieved after 5 min extraction.

Citations

Apr 2, 2011·Bioanalysis·Kihwan ChoiDoo Soo Chung
Nov 21, 2014·Bioanalysis·Maryam RezazadehShahram Seidi
Nov 14, 2006·Journal of Chromatography. a·Li XuHian Kee Lee
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Dec 19, 2006·Journal of Mass Spectrometry : JMS·Johanna M Dela CruzMarcos Dantus
Oct 15, 2011·Analytica Chimica Acta·Archana Jain, Krishna K Verma
Sep 26, 2015·Analytical and Bioanalytical Chemistry·Jihye KimDoo Soo Chung

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