Single Gold Nanoparticle-Based Colorimetric Detection of Picomolar Mercury Ion with Dark-Field Microscopy

Analytical Chemistry
Xiaojun LiuHongwei Gai

Abstract

Mercury severely damages the environment and human health, particularly when it accumulates in the food chain. Methods for the colorimetric detection of Hg(2+) have increasingly been developed over the past decade because of the progress in nanotechnology. However, the limits of detection (LODs) of these methods are mostly either comparable to or higher than the allowable maximum level (10 nM) in drinking water set by the US Environmental Protection Agency. In this study, we report a single Au nanoparticle (AuNP)-based colorimetric assay for Hg(2+) detection in solution. AuNPs modified with oligonucleotides were fixed on the slide. The fixed AuNPs bound to free AuNPs in the solution in the presence of Hg(2+) because of oligonucleotide hybridization. This process was accompanied by a color change from green to yellow as observed under an optical microscope. The ratio of changed color spots corresponded with Hg(2+) concentration. The LOD was determined as 1.4 pM, which may help guard against mercury accumulation. The proposed approach was applied to environmental samples with recoveries of 98.3 ± 7.7% and 110.0 ± 8.8% for Yuquan River and industrial wastewater, respectively.

References

Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·P J Hagerman
May 9, 2008·Chemical Communications : Chem Comm·Chi-Wei LiuHuan-Tsung Chang
Feb 4, 2009·Analytical and Bioanalytical Chemistry·Marc R Knecht, Manish Sethi
Mar 17, 2010·Journal of the American Chemical Society·Weihai NiHongkai Wu
Oct 11, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Mirna DayeMariam Hamze
Dec 3, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Huanan GuanJinzhong Liang
Aug 26, 2014·ACS Applied Materials & Interfaces·Ling ChenLingxin Chen
Sep 23, 2014·Biosensors & Bioelectronics·Liguang XuChuanlai Xu
Dec 3, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Qing WangJingjing Wang
Jan 7, 2015·Chemical Communications : Chem Comm·Xingbo ShiHongwei Gai
Jan 31, 2015·Biosensors & Bioelectronics·Wenjing YanJianhao Zhang
Dec 15, 2015·Physical Chemistry Chemical Physics : PCCP·Itai Y Stein, Brian L Wardle

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Citations

Aug 25, 2016·Scientific Reports·Weiwei ChenJilie Kong
Jan 20, 2018·International Journal of Analytical Chemistry·Lakshmi Narayana Suvarapu, Sung-Ok Baek
Sep 30, 2017·Chemical Society Reviews·Rasel DasSeeram Ramakrishna
Oct 30, 2019·Journal of the American Society for Mass Spectrometry·Hani Nasser Abdelhamid, Hui-Fen Wu
Jan 18, 2020·Nature Communications·Aniruddha RayAydogan Ozcan
Aug 24, 2017·Chemistry : a European Journal·Biswarup Jash, Jens Müller
Dec 9, 2020·Chemical Society Reviews·Verónica Montes-GarcíaPaolo Samorì
Oct 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Weizheng WangSundaram Gunasekaran

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