Oct 23, 2012

Single layer centrifugation (SLC) improves sperm quality of cryopreserved Blanca-Celtibérica buck semen

Animal Reproduction Science
Pilar Jiménez-RabadánA J Soler

Abstract

The aim of the present study was to evaluate the effect of sperm selection by means of single layer centrifugation (SLC) on sperm quality after cryopreservation, either when SLC is used before freezing or after thawing, using Blanca-Celtibérica buck semen collected by electroejaculation (EE). Ejaculates from six bucks were collected by EE and divided into two aliquots. One of them (unselected) was diluted with Biladyl(®) by the two-step method and frozen over nitrogen vapor. The other aliquot was selected by the SLC technique and subsequently frozen in the same way as the unselected samples (SLC before freezing). In a further treatment, two unselected straws were thawed and SLC was carried out (SLC after thawing). At thawing, sperm motility of all samples ((i) unselected; (ii) selected before freezing and (iii) selected after thawing) was evaluated by CASA. In addition, integrity of the plasma membrane, mitochondrial membrane potential, ROS production and DNA fragmentation index were assessed by flow cytometry. Most of the sperm parameters were improved (P≤0.001) in samples selected by SLC after thawing in relation to unselected or selected by SLC before freezing. The percentage of progressive motile spermatozoa was greater (86...Continue Reading

  • References26
  • Citations16

References

Mentioned in this Paper

Fertility
Buffers
Seminal Fluid
Flow Cytometry
Sperm Concentration Measurement
DNA Fragmentation
Hydrogen Peroxide
Physiologic Pulse
Dimethyl Sulfoxide
Maca

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