Single Layer Centrifugation with 20% or 30% Porcicoll separates the majority of spermatozoa from a sample without adversely affecting sperm quality.

Reproduction in Domestic Animals = Zuchthygiene
Sourabh DeoriJane Morrell

Abstract

Centrifugation of boar semen through one layer of 40% colloid (Porcicoll) was previously shown to separate spermatozoa from bacteria without having a detrimental effect on sperm quality. However, some spermatozoa were lost. The purpose of the present study was to determine whether 20% or 30% Porcicoll could be used to recover most of the spermatozoa without impacting on sperm quality. Insemination doses (n = 10) from a commercial boar station were sent to the laboratory at the Swedish University of Agricultural Sciences and processed by Single Layer Centrifugation with 20% and 30% Porcicoll approximately 7 hr after semen collection. The resulting sperm samples and controls were evaluated for sperm quality immediately and again after storage at 16-18°C for 4 and 7 days. Sperm recovery was 94 ± 18% and 87 ± 15% for 20% and 30% Porcicoll, respectively (p > .05). Sperm mitochondrial membrane potential and chromatin integrity were unaffected (p > .05). The proportion of live spermatozoa producing superoxide (9 ± 8%, 7 ± 6% and 3 ± 1%; p < .05), and the proportion of spermatozoa with high stainability DNA (0.68 ± 19%, 0.61 ± 0.22% and 0.96 ± 0.23%; p < .05- <0.01), were marginally increased whereas membrane integrity, although high, ...Continue Reading

References

Mar 27, 2001·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·D Evenson, L Jost
Apr 13, 2005·Theriogenology·Gry B Boe-HansenPreben Christensen
Sep 9, 2008·Theriogenology·G R FoxcroftW T Dixon
Jan 1, 2011·ISRN Veterinary Science·Marjet van WienenJane M Morrell
Dec 30, 2018·Theriogenology·J M MorrellF Martínez-Pastor

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Citations

Feb 22, 2021·Theriogenology·F Martínez-PastorJ M Morrell

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