Single measurement detection of individual cell ionic oscillations using an n-type semiconductor - electrolyte interface

Scientific Reports
Mariusz PietruszkaEdward Rówiński

Abstract

Pollen tubes are used as models in studies on the type of tip-growth in plants. They are an example of polarised and rapid growth because pollen tubes are able to quickly invade the flower pistil in order to accomplish fertilisation. How different ionic fluxes are perceived, processed or generated in the pollen tube is still not satisfactorily understood. In order to measure the H+, K+, Ca2+ and Cl- fluxes of a single pollen tube, we developed an Electrical Lab on a Photovoltaic-Chip (ELoPvC) on which the evolving cell was immersed in an electrolyte of a germination medium. Pollen from Hyacinthus orientalis L. was investigated ex vivo. We observed that the growing cell changed the (redox) potential in the medium in a periodic manner. This subtle measurement was feasible due to the effects that were taking place at the semiconductor-liquid interface. The experiment confirmed the existence of the ionic oscillations that accompany the periodic extension of pollen tubes, thereby providing - in a single run - the complete discrete frequency spectrum and phase relationships of the ion gradients and fluxes, while all of the metabolic and enzymatic functions of the cell life cycle were preserved. Furthermore, the global 1/fα characteri...Continue Reading

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Oct 9, 2015·PloS One·Mariusz Pietruszka, Aleksandra Haduch-Sendecka
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Citations

Mar 27, 2020·European Biophysics Journal : EBJ·Mariusz Pietruszka, Monika Olszewska
Sep 14, 2017·Journal of Biological Physics·Marcin Lipowczan, Mariusz Pietruszka
Apr 3, 2021·Communicative & Integrative Biology·Mariusz A Pietruszka
Apr 9, 2021·Scientific Reports·Mariusz A Pietruszka

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Software Mentioned

ELoPvC

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