Single mechanically-gated cation channel currents can trigger action potentials in neocortical and hippocampal pyramidal neurons

Brain Research
Yury A NikolaevO P Hamill

Abstract

The mammalian brain is a mechanosensitive organ that responds to different mechanical forces ranging from intrinsic forces implicated in brain morphogenesis to extrinsic forces that can cause concussion and traumatic brain injury. However, little is known of the mechanosensors that transduce these forces. In this study we use cell-attached patch recording to measure single mechanically-gated (MG) channel currents and their affects on spike activity in identified neurons in neonatal mouse brain slices. We demonstrate that both neocortical and hippocampal pyramidal neurons express stretch-activated MG cation channels that are activated by suctions of ~25mm Hg, have a single channel conductance for inward current of 50-70pS and show weak selectivity for alkali metal cations (i.e., Na(+)<K(+)<Cs(+)). Significantly, single MG channel currents activated on the soma trigger spiking/action potentials in both neocortical and hippocampal pyramidal neurons. Not all neuron types studied here expressed MG channel currents. In particular, locus coeruleus and cerebellar Purkinje neurons showed no detectable MG channel activity. Moreover their robust rhythmic spike activity was resistant to mechanical modulation. Our observation that a single ...Continue Reading

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Citations

Dec 24, 2019·Frontiers in Cellular Neuroscience·Luke R JohnsonBoris Martinac
Dec 29, 2019·Proceedings of the National Academy of Sciences of the United States of America·Benjamin M GaubDaniel J Müller
Dec 2, 2020·Neurobiology of Disease·Carolyn E Keating, D Kacy Cullen

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