Single molecule adsorption at compositionally patterned self-assembled monolayers on gold: role of domain boundaries

Langmuir : the ACS Journal of Surfaces and Colloids
Hye-Young ParkMarc D Porter

Abstract

This paper examines the single-molecule adsorption of YOYO-I-labeled lambda-DNA at compositionally patterned self-assembled monolayers (SAMs). The interactions of fluorescently labeled lambda-DNA molecule with the patterned SAMs, which are comprised of different functional groups (i.e., amine-, alcohol-, and acid-terminated thiolates), were monitored at optically transparent gold films using total internal reflection fluorescence microscopy. The role of solution pH, lambda-DNA concentration, and domain size was investigated. In addition to delineation of the relative adsorption strength as a function of terminal group identity (NH2 > COOH > OH), the potential importance of structural defects was also revealed. The latter result, found both at the disordered boundaries between domains and at adlayers in which structural order was affected by the length of the alkyl chain, points to the subtle but preferential adsorption of the "sticky ends" of lambda-DNA. These experiments also detected an intriguing dependence of adsorption with respect to domain size.

References

Apr 29, 1994·Science·R SinghviD E Ingber
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MrksichG M Whitesides
May 30, 1997·Science·C S ChenD E Ingber
Dec 18, 2001·Journal of Biomedical Materials Research·Colin A ScotchfordSandra Downes
Jul 28, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Sonny S MarkCarl A Batt
Feb 9, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Vivek P RautRobert A Latour
May 14, 2005·Analytical Chemistry·Hung-Wing LiEdward S Yeung
Aug 13, 2005·Biomaterials·Meher AntiaViola Vogel
Jul 21, 2006·The Journal of Physical Chemistry. B·Tao YeEric Borguet

❮ Previous
Next ❯

Citations

May 24, 2012·The Analyst·Robert WalderDaniel K Schwartz
Jun 8, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Eric M Peterson, Joel M Harris
Apr 7, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Zi LiDaniel A Higgins
May 23, 2008·Analytical Chemistry·Mark LowryIsiah M Warner
Dec 8, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Youngsik ChoiJaegeun Noh
Dec 17, 2009·Analytical Chemistry·Changbei Ma, Edward S Yeung
Dec 18, 2009·Analytical Chemistry·Changbei Ma, Edward S Yeung
Aug 27, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Jack C RifeLloyd J Whitman

❮ Previous
Next ❯

Related Concepts

Related Feeds

AFM in situ DNA

AFM in situ DNA describes in situ analysis (or study) of DNA using atomic force microscopy. Discover the latest research on AFM in situ DNA here.