Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum.

Nature Communications
Shenglin CaiJoshua B Edel

Abstract

MicroRNAs (miRNAs) play essential roles in post-transcriptional gene expression and are also found freely circulating in bodily fluids such as blood. Dysregulated miRNA signatures have been associated with many diseases including cancer, and miRNA profiling from liquid biopsies offers a promising strategy for cancer diagnosis, prognosis and monitoring. Here, we develop size-encoded molecular probes that can be used for simultaneous electro-optical nanopore sensing of miRNAs, allowing for ultrasensitive, sequence-specific and multiplexed detection directly in unprocessed human serum, in sample volumes as small as 0.1 μl. We show that this approach allows for femtomolar sensitivity and single-base mismatch selectivity. We demonstrate the ability to simultaneously monitor miRNAs (miR-141-3p and miR-375-3p) from prostate cancer patients with active disease and in remission. This technology can pave the way for next generation of minimally invasive diagnostic and companion diagnostic tests for cancer.

References

Jan 18, 2005·Nucleic Acids Research·Gábor E TusnádyTamás Arányi
Mar 23, 2005·Nature Methods·J Michael ThomsonScott M Hammond
Dec 22, 2005·Nature Methods·Lori A NeelyJenny Rooke
Aug 28, 2007·Nano Letters·Guillaume A T ChansinJoshua B Edel
Jul 1, 2008·Gynecologic Oncology·Douglas D Taylor, Cicek Gercel-Taylor
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Aug 19, 2008·Biophysical Journal·Meni WanunuAmit Meller
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder
Apr 8, 2009·Nature Nanotechnology·James ClarkeHagan Bayley
Dec 22, 2009·Nature Nanotechnology·Meni WanunuAmit Meller
Feb 12, 2010·Methods : a Companion to Methods in Enzymology·Evan M KrohMuneesh Tewari
Apr 24, 2010·Journal of the National Cancer Institute·H Gilbert Welch, William C Black
May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Jan C BraseHolger Sültmann
Jun 11, 2010·Annual Review of Biochemistry·Marc Robert FabianWitold Filipowicz
Sep 18, 2010·Clinical Chemistry·Jessica A WeberKai Wang
Oct 26, 2010·Nature Nanotechnology·Meni WanunuMarija Drndić
Apr 14, 2011·Clinical Chemistry·Jennifer S McDonaldAlicia Algeciras-Schimnich
Sep 6, 2011·Nature Nanotechnology·Yong WangLi-Qun Gu
Apr 19, 2012·Nature Reviews. Genetics·Colin C PritchardMuneesh Tewari
Aug 28, 2012·Trends in Biochemical Sciences·Stefan Rüegger, Helge Großhans
Sep 20, 2012·Chemical Society Reviews·Benjamin N MilesJoshua B Edel
May 25, 2013·Journal of the National Cancer Institute·Yuji ToiyamaAjay Goel
Jun 5, 2013·Indian Journal of Surgical Oncology·Mohan Adhyam, Anish Kumar Gupta
Oct 16, 2013·Scientific Reports·Timothy K K KamanuVladimir B Bajic
Oct 7, 2016·Faraday Discussions·Raquel L FraccariTim Albrecht
Apr 6, 2017·Nature Communications·Andreas C Fröbius, Peter Funch
Jun 24, 2017·Nucleic Acids Research·Limor CohenDavid R Walt
Dec 21, 2017·Seminars in Cell & Developmental Biology·Kazuharu KaiSubrata Sen
May 19, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ahmed Hussein ZedanPalle Jörn Sloth Osther
Jul 18, 2018·International Urology and Nephrology·Paweł PorzyckiMirosław Tyrka
Dec 14, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Vivek Priy DaveDang Duong Bang
Feb 24, 2019·Nature Reviews. Clinical Oncology·Klaus Pantel, Catherine Alix-Panabières
May 10, 2019·World Journal of Oncology·Prashanth Rawla
May 18, 2019·European Urology Oncology·Semini SumanasuriyaJohann S de Bono

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Citations

Jul 30, 2021·Advanced Materials·Ren RenJoshua B Edel

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