Single-molecule analysis of subtelomeres and telomeres in Alternative Lengthening of Telomeres (ALT) cells.

BMC Genomics
Heba Z AbidMing Xiao

Abstract

Telomeric DNA is typically comprised of G-rich tandem repeat motifs and maintained by telomerase (Greider CW, Blackburn EH; Cell 51:887-898; 1987). In eukaryotes lacking telomerase, a variety of DNA repair and DNA recombination based pathways for telomere maintenance have evolved in organisms normally dependent upon telomerase for telomere elongation (Webb CJ, Wu Y, Zakian VA; Cold Spring Harb Perspect Biol 5:a012666; 2013); collectively called Alternative Lengthening of Telomeres (ALT) pathways. By measuring (TTAGGG) n tract lengths from the same large DNA molecules that were optically mapped, we simultaneously analyzed telomere length dynamics and subtelomere-linked structural changes at a large number of specific subtelomeric loci in the ALT-positive cell lines U2OS, SK-MEL-2 and Saos-2. Our results revealed loci-specific ALT telomere features. For example, while each subtelomere included examples of single molecules with terminal (TTAGGG) n tracts as well as examples of recombinant telomeric single molecules, the ratio of these molecules was subtelomere-specific, ranging from 33:1 (19p) to 1:25 (19q) in U2OS. The Saos-2 cell line shows a similar percentage of recombinant telomeres. The frequency of recombinant subtelomeres ...Continue Reading

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H BiessmannJ M Mason
Jul 1, 1997·International Journal of Urology : Official Journal of the Japanese Urological Association·Y KageyamaH Oshima
Dec 2, 2000·Nature Genetics·M A DunhamR R Reddel
Apr 5, 2005·Cancer Research·Robert A MarciniakF Brad Johnson
May 12, 2007·The EMBO Journal·Andreas MeierStephen P Jackson
Oct 30, 2007·Molecular Cell·Lifeng Xu, Elizabeth H Blackburn
Dec 18, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·James M MasonHarald Biessmann
Mar 31, 2010·Nature Reviews. Genetics·Anthony J Cesare, Roger R Reddel
Apr 3, 2010·Genes & Development·Sabrina Pobiega, Stéphane Marcand
Apr 8, 2010·Cell·Teresa DavoliTitia de Lange
Aug 25, 2010·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Oumar SamassekouJu Yan
Dec 14, 2011·EMBO Reports·Zeenia KaulRoger R Reddel
May 5, 2012·Science·Agnel Sfeir, Titia de Lange
Jun 12, 2012·Aging·Elsa Vera, Maria A Blasco
Dec 12, 2012·The Journal of Cell Biology·Dimitri ConomosHilda A Pickett
Jun 5, 2013·Cold Spring Harbor Perspectives in Biology·Christopher J WebbVirginia A Zakian
Dec 18, 2013·Neoplasia : an International Journal for Oncology Research·Despoina SakellariouSarantis Gagos
Oct 22, 2014·Nature Communications·Rajika AroraClaus M Azzalin
Nov 20, 2015·Nature Structural & Molecular Biology·Hilda A Pickett, Roger R Reddel
Nov 4, 2016·Nature·Robert L DilleyRoger A Greenberg
Jan 14, 2017·Science·Julia Su Zhou LiEros Lazzerini Denchi
Jan 18, 2017·Nature Reviews. Molecular Cell Biology·John Maciejowski, Titia de Lange
Jul 1, 2017·Cell·Hsueh-Ping ChuJeannie T Lee
Sep 8, 2017·The EMBO Journal·Alexander P SobinoffHilda A Pickett
Oct 4, 2017·Trends in Genetics : TIG·Alexander P Sobinoff, Hilda A Pickett
Oct 14, 2017·Genome Research·Jennifer McCaffreyMing Xiao
Jan 28, 2020·PLoS Genetics·Eleanor YoungMing Xiao

❮ Previous
Next ❯

Citations

Nov 25, 2020·Nucleic Acids Research·Heba Z AbidMing Xiao
Mar 20, 2021·Essays in Biochemistry·Jonathan JeffetYuval Ebenstein
Jul 8, 2021·Analytical Chemistry·Lahari UppuluriMing Xiao

❮ Previous
Next ❯

Software Mentioned

Refaligner
Assembler

Related Concepts

Related Feeds

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

Related Papers

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
Oumar SamassekouJu Yan
Expert Review of Gastroenterology & Hepatology
Nivedita BasuLisa A Boardman
© 2021 Meta ULC. All rights reserved