Single-molecule DNA analysis

Annual Review of Analytical Chemistry
J William Efcavitch, John F Thompson

Abstract

The ability to detect single molecules of DNA or RNA has led to an extremely rich area of exploration of the single most important biomolecule in nature. In cases in which the nucleic acid molecules are tethered to a solid support, confined to a channel, or simply allowed to diffuse into a detection volume, novel techniques have been developed to manipulate the DNA and to examine properties such as structural dynamics and protein-DNA interactions. Beyond the analysis of the properties of nucleic acids themselves, single-molecule detection has enabled dramatic improvements in the throughput of DNA sequencing and holds promise for continuing progress. Both optical and nonoptical detection methods that use surfaces, nanopores, and zero-mode waveguides have been attempted, and one optically based instrument is already commercially available. The breadth of literature related to single-molecule DNA analysis is vast; this review focuses on a survey of efforts in molecular dynamics and nucleic acid sequencing.

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Citations

Oct 13, 2012·Chemical Reviews·Ekaterina M Nestorovich, Sergey M Bezrukov
Sep 14, 2013·Journal of Microbiological Methods·Julia M Di BellaGregor Reid
May 12, 2012·Current Opinion in Structural Biology·Jonas Korlach, Stephen W Turner
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Apr 10, 2013·The Journal of Physical Chemistry. B·Guihua WangXiyun Guan
Mar 7, 2014·ACS Nano·Hui YuNongjian Tao
Oct 5, 2010·Current Protocols in Molecular Biology·John F Thompson, Kathleen E Steinmann
Feb 26, 2011·Genome Biology·John F Thompson, Patrice M Milos
Aug 7, 2020·Nucleic Acids Research·Yiwen LiPhilipp Kukura

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Methods Mentioned

BETA
electron microscopy
FRET
atomic force microscopy
AFM
optical tweezer
scanning tunneling spectroscopy
scanning tunneling microscopy
biosensors

Software Mentioned

HeliScope

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