Single molecule fate of HIV-1 envelope reveals late-stage viral lattice incorporation

Nature Communications
Carmen A ButtlerSchuyler B van Engelenburg

Abstract

Human immunodeficiency virus type 1 (HIV-1) assembly occurs on the inner leaflet of the host cell plasma membrane, incorporating the essential viral envelope glycoprotein (Env) within a budding lattice of HIV-1 Gag structural proteins. The mechanism by which Env incorporates into viral particles remains poorly understood. To determine the mechanism of recruitment of Env to assembly sites, we interrogate the subviral angular distribution of Env on cell-associated virus using multicolor, three-dimensional (3D) superresolution microscopy. We demonstrate that, in a manner dependent on cell type and on the long cytoplasmic tail of Env, the distribution of Env is biased toward the necks of cell-associated particles. We postulate that this neck-biased distribution is regulated by vesicular retention and steric complementarity of Env during independent Gag lattice formation.

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C F BarbasE Norrby
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R BurtonR A Lerner
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H G GöttlingerW A Haseltine
Aug 1, 1989·Journal of Medical Virology·M TremblayM A Wainberg
Apr 5, 1993·Journal of Molecular Biology·C F BarbasG R Pilkington
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·T Murakami, E O Freed
Apr 23, 2002·Journal of Virology·Eric O Freed
Dec 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ping ZhuKenneth H Roux
Oct 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Helge EwersAri Helenius
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Jul 22, 2008·Nature Methods·Khuloud JaqamanGaudenz Danuser
Jul 23, 2008·Angewandte Chemie·Mike HeilemannMarkus Sauer
Feb 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Gleb ShtengelHarald F Hess
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Wesley I Sundquist, Hans-Georg Kräusslich
Jan 18, 2014·Science·Schuyler B Van EngelenburgJennifer Lippincott-Schwartz
Jun 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Mingli QiPaul Spearman
Jun 30, 2015·Nature Reviews. Microbiology·Eric O Freed
Dec 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Philip R TedburyEric O Freed
Apr 28, 2016·FEBS Letters·Janina HanneHans-Georg Kräusslich
Nov 1, 2016·Nature Medicine·Peter RusertUNKNOWN Swiss HIV Cohort Study
Apr 28, 2017·Journal of Virology·Armando StanoMichael B Zwick
Sep 17, 2017·Nature Communications·Jakub ChojnackiChristian Eggeling

❮ Previous
Next ❯

Citations

Nov 24, 2019·Proceedings of the National Academy of Sciences of the United States of America·Nairi PezeshkianSchuyler B van Engelenburg
Jul 16, 2020·Viruses·Prabuddha Sengupta, Jennifer Lippincott-Schwartz
Oct 14, 2018·Nature Reviews. Microbiology·Christof Hepp, Nicole C Robb
May 16, 2020·Frontiers in Bioengineering and Biotechnology·Mai IzumidaYoshinao Kubo
Apr 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexej Dick, Simon Cocklin
Sep 15, 2019·The Journal of Biological Chemistry·Huxley K HoffmanSchuyler B van Engelenburg
Jul 4, 2018·Retrovirology·UNKNOWN Retrovirology Editorial
Dec 18, 2020·Nature Protocols·Jingyu WangJoerg Bewersdorf

❮ Previous
Next ❯

Methods Mentioned

BETA
superresolution microscopy
transmission electron microscopy
confocal microscopy
fluorescence recovery after photobleaching
myristoylation
PCA

Software Mentioned

MATLAB
CEM
uTrack
ImageJ
iPALM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.