Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function

Biophysics Reviews
Mark HelmG Ulrich Nienhaus

Abstract

Single-molecule fluorescence microscopy experiments on RNA molecules brought to light the highly complex dynamics of key biological processes, including RNA folding, catalysis of ribozymes, ligand sensing of riboswitches and aptamers, and protein synthesis in the ribosome. By using highly advanced biophysical spectroscopy techniques in combination with sophisticated biochemical synthesis approaches, molecular dynamics of individual RNA molecules can be observed in real time and under physiological conditions in unprecedented detail that cannot be achieved with bulk experiments. Here, we review recent advances in RNA folding and functional studies of RNA and RNA-protein complexes addressed by using single-molecule Förster (fluorescence) resonance energy transfer (smFRET) technique.

References

Jan 1, 1995·Annual Review of Biochemistry·L GoldM Yarus
Apr 1, 1997·Current Opinion in Structural Biology·J Frank
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y JiaR M Hochstrasser
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·P Brion, E Westhof
Jan 8, 1999·Journal of Molecular Biology·A SytnikR M Hochstrasser
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·B ChanK Musier-Forsyth
Mar 13, 1999·Chemistry & Biology·B Seelig, A Jäschke
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·G A Soukup, R R Breaker
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·T HaS Chu
Dec 3, 1999·Nature Structural Biology·X W FangT R Sosnick
May 2, 2000·Journal of Molecular Biology·G A SoukupR R Breaker
Jun 17, 2000·Science·X ZhuangS Chu
Jun 29, 2000·Annual Review of Biochemistry·D S Wilson, J W Szostak
Oct 4, 2000·Biochemistry·S K SilvermanT R Cech
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·E KauffmannK Gerwert
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rick RussellDaniel Herschlag
Feb 19, 2002·Current Opinion in Biotechnology·Ronald R Breaker
Mar 23, 2002·Cell·V Ramakrishnan
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Harold D KimSteven Chu
Feb 13, 2003·Current Opinion in Structural Biology·Xiaowei Zhuang, Matthias Rief
Mar 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth RhoadesGilad Haran
Jul 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gregory BokinskyXiaowei Zhuang
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elliot TanTaekjip Ha
Aug 30, 2003·Science·Everett A LipmanWilliam A Eaton
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zheng XieNorbert F Scherer
Jan 27, 2004·Journal of Molecular Biology·Sungchul HohngTaekjip Ha
May 14, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Elza V AmirgoulovaG Ulrich Nienhaus
Jun 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·David RuedaNils G Walter
Aug 10, 2004·Biophysical Journal·Sungchul HohngTaekjip Ha
Aug 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Scott C BlanchardSteven Chu
Sep 28, 2004·Nature Structural & Molecular Biology·Scott C BlanchardJoseph D Puglisi
Oct 12, 2004·Nature Structural & Molecular Biology·Michelle K NahasTaekjip Ha
Jan 14, 2005·Nucleic Acids Research·Magdalena DorywalskaJoseph D Puglisi
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Benjamin SchulerWilliam A Eaton
Mar 18, 2005·Journal of the American Chemical Society·Mike HeilemannPhilip Tinnefeld
Mar 24, 2005·Physical Review Letters·Mark BatesXiaowei Zhuang
Mar 30, 2005·Biochemistry·D Thirumalai, Changbong Hyeon

❮ Previous
Next ❯

Citations

Oct 29, 2013·Protoplasma·Per Niklas Hedde, G Ulrich Nienhaus
Dec 1, 2012·Biophysics Reviews·Li Shang, G Ulrich Nienhaus
Aug 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shangguo HouKevin Welsher
Jul 3, 2021·Nano Letters·Nadav OpatovskiYoav Shechtman
Aug 20, 2021·The Journal of Organic Chemistry·Anna-Lena Johanna Segler, Snorri Th Sigurdsson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Annual Review of Biophysics and Biomolecular Structure
Xiaowei Zhuang
Methods in Enzymology
Bassem SheblPeter V Cornish
Methods : a Companion to Methods in Enzymology
Rui Zhao, David Rueda
Journal of the American Chemical Society
Bishnu P Paudel, David Rueda
© 2022 Meta ULC. All rights reserved