Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids

Current Opinion in Structural Biology
Swati Tyagi, Edward A Lemke

Abstract

Contemporary structural biology research promises more than just static snap-shots of molecular machineries. This goal is not just facilitated by combining different structural biology techniques, but also by new tools from the field of protein and genetic engineering, as well as from chemistry. Genetic encoding of noncanonical amino acids (ncAAs) through codon-suppression technology provides an excellent opportunity to probe biomolecules using different structural biology methods. In this article, we review the applications of ncAA incorporation into proteins for determining structural information through various techniques with the main focus on crosslinking mass spectrometry and single-molecule FRET-based techniques. Furthermore, advances and limitations of the incorporation of multiple ncAAs are discussed, with respect to design of an ideal host organism for modern and integrative structural biology research.

References

Apr 21, 2001·Science·L WangP G Schultz
Aug 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jason W ChinPeter G Schultz
Aug 23, 2005·Biophysical Journal·Barbara K MüllerDon C Lamb
Apr 29, 2006·Science·György PósfaiFrederick R Blattner
Apr 9, 2009·Journal of the American Chemical Society·Young Jun SeoFloyd E Romesberg
Mar 24, 2010·Annual Review of Biochemistry·Chang C Liu, Peter G Schultz
Aug 13, 2010·Nucleic Acids Research·Takahito MukaiKensaku Sakamoto
Mar 25, 2011·Angewandte Chemie·Tilman PlassEdward A Lemke
Sep 6, 2011·The Journal of Physical Chemistry. B·Adam Muschielok, Jens Michaelis
Sep 20, 2011·Nature Chemical Biology·David B F JohnsonLei Wang
Nov 17, 2011·Nature Chemical Biology·Nicholas Stephanopoulos, Matthew B Francis
Feb 24, 2012·Journal of the American Chemical Society·Sigrid MillesEdward A Lemke
Apr 5, 2012·Angewandte Chemie·Tilman PlassEdward A Lemke
Sep 5, 2012·Chembiochem : a European Journal of Chemical Biology·Annika BorrmannEdward A Lemke
Sep 12, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maoqing DongLaurence J Miller
Nov 13, 2012·Nature Methods·Stanislav KalininClaus A M Seidel
Nov 14, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sigrid Milles, Edward A Lemke
Mar 21, 2013·Angewandte Chemie·Moritz J Schmidt, Daniel Summerer
Jul 10, 2013·Current Opinion in Structural Biology·William H ZhangColin J Jackson
Oct 19, 2013·Science·Marc J LajoieFarren J Isaacs
Oct 19, 2013·Science·M J LajoieG M Church
Jan 30, 2014·Angewandte Chemie·Ivana NikićEdward A Lemke
Feb 22, 2014·Annual Review of Biochemistry·Jason W Chin
Mar 25, 2014·Chemical Reviews·Kathrin Lang, Jason W Chin
May 9, 2014·Nature·Denis A MalyshevFloyd E Romesberg
Aug 1, 2014·Chembiochem : a European Journal of Chemical Biology·Edward A Lemke

❮ Previous
Next ❯

Citations

Dec 29, 2015·Journal of Molecular Biology·Ashok A Deniz
Nov 13, 2015·British Journal of Pharmacology·A D Stumpf, C Hoffmann
Aug 25, 2015·Current Opinion in Chemical Biology·Ivana Nikić, Edward A Lemke
Jun 20, 2016·Current Opinion in Structural Biology·Petra Neumann-Staubitz, Heinz Neumann
Nov 25, 2016·Biotechnology and Bioengineering·Eden OzerLital Alfonta
Jan 12, 2016·Chemical Communications : Chem Comm·Laura Graña-SuárezJurriaan Huskens
Nov 21, 2019·Chembiochem : a European Journal of Chemical Biology·Byung Joon LimGalia T Debelouchina
Jan 10, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Lena Voith von Voithenberg, Don C Lamb
Dec 25, 2019·Annual Review of Biochemistry·Mateusz DylaPoul Nissen
Oct 7, 2018·Scientific Reports·Christina S HeilMartin Grininger
Apr 27, 2016·Chembiochem : a European Journal of Chemical Biology·Taehyung C LeeAshok A Deniz
May 5, 2021·Chembiochem : a European Journal of Chemical Biology·Kim BartelsChristian Löw
Oct 24, 2020·Biophysical Journal·Ryan J DurhamVasanthi Jayaraman
Nov 17, 2020·The Journal of Physical Chemistry. B·Jimin YoonE James Petersson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.