Single-molecule imaging of NGF axonal transport in microfluidic devices.

Lab on a Chip
Kai ZhangBianxiao Cui

Abstract

Nerve growth factor (NGF) signaling begins at the nerve terminal, where it binds and activates membrane receptors and subsequently carries the cell-survival signal to the cell body through the axon. A recent study revealed that the majority of endosomes contain a single NGF molecule, which makes single-molecule imaging an essential tool for NGF studies. Despite being an increasingly popular technique, single-molecule imaging in live cells is often limited by background fluorescence. Here, we employed a microfluidic culture platform to achieve background reduction for single-molecule imaging in live neurons. Microfluidic devices guide the growth of neurons and allow separately controlled microenvironment for cell bodies or axon termini. Designs of microfluidic devices were optimized and a three-compartment device successfully achieved direct observation of axonal transport of single NGF when quantum dot labeled NGF (Qdot-NGF) was applied only to the distal-axon compartment while imaging was carried out exclusively in the cell-body compartment. Qdot-NGF was shown to move exclusively toward the cell body with a characteristic stop-and-go pattern of movements. Measurements at various temperatures show that the rate of NGF retrograd...Continue Reading

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Citations

Mar 13, 2012·Annual Review of Physical Chemistry·Praveen D ChowdaryBianxiao Cui
Feb 21, 2013·ACS Chemical Neuroscience·Allyson E SgroDaniel T Chiu
May 8, 2013·Lab on a Chip·William J PolacheckRoger D Kamm
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Sep 25, 2012·Analytical and Bioanalytical Chemistry·Wangxi LuoXiaohong Fang
Jul 10, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Wenjun XieBianxiao Cui
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Oct 4, 2012·Trends in Neurosciences·Larry J Millet, Martha U Gillette
Nov 4, 2015·Neuroscience Letters·Daphne L CheBianxiao Cui
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Mar 31, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Pazit Polak, Orit Shefi
Sep 2, 2014·Progress in Biophysics and Molecular Biology·Sebastien G M UzelRoger D Kamm
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Feb 26, 2011·Current Opinion in Neurobiology·Toh Hean Ch'ng, Kelsey C Martin
Jun 4, 2015·Biophysical Journal·Praveen D ChowdaryBianxiao Cui
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Nov 1, 2016·Protein & Cell·Mengmeng ChenJane Y Wu
Nov 2, 2019·Traffic·Sunaina SuranaGiampietro Schiavo
Apr 18, 2020·Analytical and Bioanalytical Chemistry·Min ChenYao-Qun Li
Jun 24, 2018·Biophysical Journal·Praveen D ChowdaryBianxiao Cui

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