Single-molecule investigation of the interactions between reconstituted planar lipid membranes and an analogue of the HP(2-20) antimicrobial peptide

Biochemical and Biophysical Research Communications
Loredana MereutaKyung-Soo Hahm

Abstract

In this study, we employed electrophysiology experiments carried out at the single-molecule level to study the mechanism of action of the HPA3 peptide, an analogue of the linear antimicrobial peptide, HP(2-20), isolated from the N-terminal region of the Helicobacter pylori ribosomal protein. Amplitude analysis of currents fluctuations induced by HPA3 peptide at various potentials in zwitterionic lipid membranes reveal the existence of reproducible conductive states in the stochastic behavior of such events, which directly supports the existence of transmembrane pores induced the peptide. From our data recorded both at the single-pore and macroscopic levels, we propose that the HPA3 pore formation is electrophoretically facilitated by trans-negative transmembrane potentials, and HPA3 peptides translocate into the trans monolayers after forming the pores. We present evidence according to which the decrease in the membrane dipole potential of a reconstituted lipid membranes leads to an augmentation of the membrane activity of HPA3 peptides, and propose that a lower electric dipole field of the interfacial region of the membrane caused by phloretin facilitates the surface-bound HPA3 peptides to break free from one leaflet of the me...Continue Reading

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Citations

Oct 3, 2012·PloS One·Olga S OstroumovaLudmila V Schagina
Dec 15, 2015·European Biophysics Journal : EBJ·Svetlana S EfimovaOlga S Ostroumova
Jul 16, 2014·The Journal of Membrane Biology·Svetlana S EfimovaOlga S Ostroumova
Jan 27, 2010·Biochimica Et Biophysica Acta·Tzong-Hsien LeeMarie-Isabel Aguilar
Oct 11, 2019·Environmental Science and Pollution Research International·Sang-Il HanYoon-E Choi
Aug 3, 2018·Scientific Reports·Anastasiia A ZakharovaOlga S Ostroumova
Jul 23, 2020·Frontiers in Cell and Developmental Biology·Svetlana S EfimovaOlga S Ostroumova
Nov 28, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Alina AsandeiTudor Luchian
Jun 19, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Svetlana S Efimova, Olga S Ostroumova
Dec 4, 2020·ACS Applied Materials & Interfaces·Alina AsandeiTudor Luchian
Jun 28, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Svetlana S EfimovaOlga S Ostroumova

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