Single-Molecule Localization Microscopy to Study Protein Organization in the Filamentous Fungus Trichoderma atroviride.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Alexander W A F ReismannGerhard J Schütz

Abstract

Single-molecule localization microscopy has boosted our understanding of biological samples by offering access to subdiffraction resolution using fluorescence microscopy methods. While in standard mammalian cells this approach has found wide-spread use, its application to filamentous fungi has been scarce. This is mainly due to experimental challenges that lead to high amounts of background signal because of ample autofluorescence. Here, we report the optimization of labeling, imaging and data analysis protocols to yield the first single-molecule localization microscopy images of the filamentous fungus Trichoderma atroviride. As an example, we show the spatial distribution of the Sur7 tetraspanin-family protein Sfp2 required for hyphal growth and cell wall stability in this mycoparasitic fungus.

References

Oct 25, 2006·Nature Methods·Ulrich RothbauerHeinrich Leonhardt
Jan 8, 2008·Nature Methods·Makio TokunagaKumiko Sakata-Sogawa
Jul 2, 2011·Nature Protocols·Sebastian van de LindeMarkus Sauer
Sep 17, 2011·Nature Reviews. Microbiology·Irina S DruzhininaChristian P Kubicek
Oct 3, 2013·Physical Chemistry Chemical Physics : PCCP·John R AllenMichael W Davidson
Apr 15, 2015·Nucleic Acids Research·Markus Hirsch, Mark Helm
Apr 29, 2016·Bioscience, Biotechnology, and Biochemistry·Norio Takeshita
Jan 11, 2018·Nature Communications·Anna-Karin GustavssonW E Moerner
Dec 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexander W A F ReismannGerhard J Schütz
Jan 4, 2019·Nature Cell Biology·Lothar SchermellehGregor P C Drummen
Dec 17, 2019·Journal of Visualized Experiments : JoVE·Alexander Lichius, Susanne Zeilinger

❮ Previous
Next ❯

Methods Mentioned

BETA
optical microscopy

Software Mentioned

Daostorm

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.