Single-molecule spectroscopy of the temperature-induced collapse of unfolded proteins.

Proceedings of the National Academy of Sciences of the United States of America
Daniel NettelsBenjamin Schuler

Abstract

We used single-molecule FRET in combination with other biophysical methods and molecular simulations to investigate the effect of temperature on the dimensions of unfolded proteins. With single-molecule FRET, this question can be addressed even under near-native conditions, where most molecules are folded, allowing us to probe a wide range of denaturant concentrations and temperatures. We find a compaction of the unfolded state of a small cold shock protein with increasing temperature in both the presence and the absence of denaturant, with good agreement between the results from single-molecule FRET and dynamic light scattering. Although dissociation of denaturant from the polypeptide chain with increasing temperature accounts for part of the compaction, the results indicate an important role for additional temperature-dependent interactions within the unfolded chain. The observation of a collapse of a similar extent in the extremely hydrophilic, intrinsically disordered protein prothymosin alpha suggests that the hydrophobic effect is not the sole source of the underlying interactions. Circular dichroism spectroscopy and replica exchange molecular dynamics simulations in explicit water show changes in secondary structure cont...Continue Reading

References

May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E HaasI Z Steinberg
Mar 1, 1992·Protein Science : a Publication of the Protein Society·A Perczel, G D Fasman
Jul 20, 1992·Journal of Molecular Biology·G I Makhatadze, P L Privalov
Jan 1, 1991·Annual Review of Biophysics and Biophysical Chemistry·H S Chan, K A Dill
Aug 7, 1990·Biochemistry·K A Dill
Feb 12, 1996·FEBS Letters·A NöppertG Damaschun
Jun 1, 1996·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·S S PlotkinP G Wolynes
Mar 27, 1999·Journal of Molecular Biology·B A Shoemaker, P G Wolynes
May 14, 1999·Current Opinion in Structural Biology·D Thirumalai, D K Klimov
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·A A DenizS Weiss
Apr 17, 2002·Physical Review Letters·Jorge ChahineJosé Nelson Onuchic
Jul 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhengshuang ShiNeville R Kallenbach
Nov 7, 2002·Advances in Protein Chemistry·Ian S MillettKevin W Plaxco
Oct 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mourad SadqiVictor Muñoz
Dec 25, 2003·Journal of the American Chemical Society·Wei Yuan YangMartin Gruebele
Jan 20, 2004·Journal of Molecular Biology·Christine Magg, Franz X Schmid
Jul 23, 2004·The Journal of Chemical Physics·Hans W HornTeresa Head-Gordon
Feb 5, 2005·Trends in Biochemical Sciences·Nicholas C FitzkeeGeorge D Rose
Nov 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ted A LaurenceShimon Weiss
Jan 24, 2006·Journal of Molecular Biology·Elza V KuzmenkinaG Ulrich Nienhaus
May 11, 2006·Chemical Reviews·Xavier MichaletMarcus Jäger
Jun 27, 2006·Biophysical Journal·Tomoko Tezuka-KawakamiD Alastair Smith
Jul 4, 2006·Journal of Molecular Biology·Christine MaggFranz X Schmid
Jul 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eilon Sherman, Gilad Haran
Aug 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Andreas MöglichThomas Kiefhaber
Dec 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Armin HoffmannBenjamin Schuler
Jan 26, 2007·Current Opinion in Structural Biology·Tanja Mittag, Julie D Forman-Kay
Jan 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kusai A MerchantWilliam A Eaton
Feb 13, 2007·Journal of Molecular Biology·Jaby JacobTobin R Sosnick
Feb 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel NettelsBenjamin Schuler
Apr 21, 2007·Journal of Fluorescence·Frank HillgerBenjamin Schuler
Nov 7, 2007·Journal of the American Chemical Society·Troy CellmerWilliam A Eaton
Jan 1, 2008·Journal of Molecular Biology·Hagen HofmannRenate Ulbrich-Hofmann
Jan 29, 2008·Current Opinion in Structural Biology·Benjamin Schuler, William A Eaton
Jun 4, 2008·Annual Review of Biochemistry·D Wayne Bolen, George D Rose
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Edward P O'BrienD Thirumalai

❮ Previous
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Citations

Jan 2, 2013·Journal of Biological Physics·Antonio BianconiGaetano Campi
Aug 23, 2011·Journal of the American Chemical Society·Anna BerteottiMichele Parrinello
Feb 24, 2012·Journal of the American Chemical Society·Sigrid MillesEdward A Lemke
Sep 10, 2013·Journal of the American Chemical Society·Mikayel AznauryanBenjamin Schuler
Jun 21, 2011·Physical Chemistry Chemical Physics : PCCP·Stefan BollmannSören Doose
Jul 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sonja Müller-SpäthBenjamin Schuler
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrea SorannoBenjamin Schuler
Aug 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rahul K Das, Rohit V Pappu
Jul 28, 2012·International Journal of Molecular Sciences·Ming HaoGuohui Li
Jan 18, 2014·Chemical Reviews·Marco BrucaleBruno Samorì
Sep 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hagen HofmannBenjamin Schuler
Oct 5, 2013·The Journal of Chemical Physics·Hagen HofmannBenjamin Schuler
Mar 1, 2013·The Journal of Chemical Physics·Ryan R ChengDmitrii E Makarov
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·René WuttkeBenjamin Schuler
Mar 20, 2014·Quarterly Reviews of Biophysics·Hannah Gelman, Martin Gruebele
Jan 12, 2011·Annual Review of Physical Chemistry·Jeremy L England, Gilad Haran
Dec 20, 2015·Protein Science : a Publication of the Protein Society·Maodong Li, Zhirong Liu
Jun 20, 2015·Protein Science : a Publication of the Protein Society·Gi-Moon Nam, Dmitrii E Makarov
Jan 20, 2012·Current Opinion in Structural Biology·Robert B Best
Nov 23, 2011·Current Opinion in Structural Biology·Gilad Haran
Mar 1, 2016·Chemical Reviews·Veronika CsizmokJulie D Forman-Kay
Feb 10, 2011·Biochimica Et Biophysica Acta·Allan Chris M Ferreon, Ashok A Deniz
Dec 15, 2010·Current Opinion in Structural Biology·Tobin R Sosnick, Doug Barrick
Oct 9, 2014·Biophysical Journal·Gül H ZerzeJeetain Mittal
Aug 19, 2015·Journal of Molecular Biology·Lipi ThukralHannes Neuweiler
Apr 3, 2012·Protein Science : a Publication of the Protein Society·Lei Huang, Eugene I Shakhnovich
Nov 18, 2015·Journal of Chemical Theory and Computation·Wenwei ZhengRobert B Best
Nov 3, 2015·The Journal of Physical Chemistry. B·Athi N Naganathan, David De Sancho
Oct 27, 2015·The Journal of Physical Chemistry. B·Gül H ZerzeJeetain Mittal
Jan 15, 2013·Current Opinion in Structural Biology·Benjamin Schuler, Hagen Hofmann
Dec 18, 2012·The Biochemical Journal·Albert H MaoRohit V Pappu
Apr 8, 2016·The Journal of Physical Chemistry. B·Mark S MillerAdrian Hamilton Elcock
Apr 19, 2011·Journal of Molecular Biology·Daniel P TeufelHannes Neuweiler
Oct 4, 2011·Biophysical Journal·Sigrid Milles, Edward A Lemke
Jan 10, 2012·Biophysical Journal·Carlo CamilloniAlfonso De Simone

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