Single-Molecule Spectroscopy Study of Crowding-Induced Protein Spontaneous Denature and Crowding-Perturbed Unfolding-Folding Conformational Fluctuation Dynamics

The Journal of Physical Chemistry. B
Zijiang Wang, H Peter Lu

Abstract

The effects of molecular crowding on protein folding-unfolding processes are of importance for understanding protein function and structure dynamics in living cells. The enhancement of protein stability as a result of reduced entropic effect in the presence of molecular crowding is well understood both experimentally and theoretically. However, because of the complexity and interplay between various interactions existing in an equally favored environment of protein folding and unfolding conformational dynamics, such a simple reduced entropic enhancement model does not suffice to describe protein folding conformational dynamics under a protein crowding condition. In this paper, we report our observation on that single protein molecules spontaneously denature into unfolded proteins and folding-unfolding fluctuations in solution of crowding reagent Ficoll 70. We have identified that such protein dynamics involves a combined mechanism of polymer-polymer interaction, entropic effects, and protein solvation dynamics. We characterize the protein folding-unfolding dynamics by using single-molecule spectroscopy to obtain detailed molecular dynamic scale information on the protein folding-unfolding conformational fluctuation dynamics. Ou...Continue Reading

References

Jul 20, 1992·Journal of Molecular Biology·G I Makhatadze, P L Privalov
Dec 20, 1992·Journal of Molecular Biology·R ChattopadhyayaF A Quiocho
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R Zwanzig
May 2, 1985·Nature·Y S BabuW J Cook
Dec 4, 1984·Biochemistry·T Arakawa, S N Timasheff
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·S B Zimmerman, A P Minton
Jan 1, 1995·Trends in Biochemical Sciences·P JamesE Carafoli
Jan 1, 1997·Nature Structural Biology·K A Dill, H S Chan
Jan 1, 1997·Annual Review of Physical Chemistry·J N OnuchicP G Wolynes
Dec 4, 1998·Science·H P LuX S Xie
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·T HaS Weiss
May 14, 1999·Current Opinion in Structural Biology·D Thirumalai, D K Klimov
Dec 22, 1999·The EMBO Journal·B van den BergC M Dobson
Feb 19, 2000·Current Opinion in Structural Biology·A P Minton
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·A A DenizS Weiss
Jul 8, 2000·Trends in Cell Biology·D Chin, A R Means
Jul 24, 2004·Nature Medicine·Christopher A Ross, Michelle A Poirier
Oct 7, 2004·Biophysical Journal·Vitor B P LeiteJin Wang
Mar 30, 2005·Biochemistry·D Thirumalai, Changbong Hyeon
Jun 9, 2005·Methods in Molecular Biology·H Peter Lu
Jul 2, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Benjamin Schuler
Aug 25, 2005·Journal of the American Chemical Society·Brian D SlaughterCarey K Johnson
Jul 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eilon Sherman, Gilad Haran
Aug 3, 2006·Journal of the American Chemical Society·Ruchuan LiuH Peter Lu
Jun 14, 2008·Biophysical Journal·Elias M PuchnerHermann E Gaub
Oct 28, 2008·The Journal of Physical Chemistry. B·Xuefei Wang, H Peter Lu
Dec 17, 2008·Physical Chemistry Chemical Physics : PCCP·Guy ZivGilad Haran
Jul 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hoi Sung ChungWilliam A Eaton
Mar 2, 2010·Nature Reviews. Drug Discovery·Adriano Aguzzi, Tracy O'Connor
Apr 8, 2010·The Journal of Physical Chemistry. B·Yuanmin Wang, H Peter Lu
Jan 25, 2011·Microbial Biotechnology·Prashanth BhagannaJohn E Hallsworth
Aug 10, 2011·Journal of the American Chemical Society·Yufan HeH Peter Lu
Oct 29, 2011·Science·Johannes StiglerMatthias Rief
Nov 23, 2011·Current Opinion in Structural Biology·Gilad Haran
Nov 22, 2012·Biochemistry·Laura A BentonGary J Pielak
Jan 3, 2013·Current Opinion in Structural Biology·Gabriel Zoldák, Matthias Rief
Apr 8, 2017·The Journal of Physical Chemistry. B·Priyanka Singh, Pramit K Chowdhury
May 5, 2017·Angewandte Chemie·Jessica Valle-OreroJulio M Fernández

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Citations

Jan 11, 2020·Essays in Biochemistry·Christian Schöneich
Jan 23, 2020·The Journal of Physical Chemistry Letters·Lauren A WarningStephan Link
May 31, 2019·The Journal of Physical Chemistry. B·Achintya KunduMinhaeng Cho
Apr 5, 2019·The Journal of Physical Chemistry. B·Atanu NandySaptarshi Mukherjee
Aug 31, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Subhasree GhoshN Prakash Prabhu

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