Single molecule studies of RNA polymerase II transcription in vitro

Transcription
Abigail E HornJennifer F Kugel

Abstract

Eukaryotic mRNA transcription by RNA polymerase II (RNAP II) is the first step in gene expression and a key determinant of cellular regulation. Elucidating the mechanism by which RNAP II synthesizes RNA is therefore vital to determining how genes are controlled under diverse biological conditions. Significant advances in understanding RNAP II transcription have been achieved using classical biochemical and structural techniques; however, aspects of the transcription mechanism cannot be assessed using these approaches. The application of single-molecule techniques to study RNAP II transcription has provided new insight only obtainable by studying molecules in this complex system one at a time.

References

Mar 30, 2001·The Journal of Biological Chemistry·J WuL J Parkhurst
Jul 25, 2006·Critical Reviews in Biochemistry and Molecular Biology·Mary C Thomas, Cheng-Ming Chiang
Nov 25, 2006·Nature Methods·Tamar Juven-GershonJames T Kadonaga
Feb 27, 2007·Cell·Bing LiJerry L Workman
Aug 8, 2007·Journal of Molecular Biology·Aaron R HiebJames A Goodrich
Nov 13, 2007·Nature Structural & Molecular Biology·Peter SchluescheMichael Meisterernst
Dec 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Joanna AndreckaJens Michaelis
Apr 16, 2008·Annual Review of Biochemistry·Kristina M HerbertSteven M Block
Oct 14, 2008·Nature Methods·Adam MuschielokJens Michaelis
Dec 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chin-Yu ChenWei-Hau Chang
Aug 1, 2009·Science·Courtney HodgesCarlos Bustamante
Nov 15, 2011·Nature Structural & Molecular Biology·Lacramioara BintuCarlos Bustamante
Mar 20, 2012·Molecular Cell·Barbara TreutleinJens Michaelis
Apr 5, 2012·Physical Biology·Daniel J BillingsleyNeil H Thomson
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Matthew H LarsonSteven M Block
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bradley ZamftCarlos Bustamante
Jul 20, 2012·Genes & Development·Andrey RevyakinRobert Tjian
Nov 13, 2012·Cell·Lacramioara BintuCarlos Bustamante
Mar 26, 2013·Nature Methods·J Christof M GebhardtX Sunney Xie
Sep 24, 2013·Chemical Reviews·Jens Michaelis, Barbara Treutlein
Sep 28, 2013·Science·Kenji MurakamiRoger D Kornberg

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Citations

Oct 29, 2020·Genome Génome / Conseil National De Recherches Canada·Rachel M PriceSheila S Teves
Mar 17, 2019·Methods : a Companion to Methods in Enzymology·Elina LyJennifer F Kugel

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