Single-molecule tracking

Methods in Molecular Biology
Marija VrljicW E Moerner

Abstract

The current models of eukaryotic plasma membrane organization separate the plasma membrane nto different environments created by lipids and interactions between membrane proteins and the cytoskeleton, but characterization of their physical properties, such as their sizes, lifetimes, and the partitioning of membrane components into each environment, has not been accomplished. Single-moleule (fluorophore) tracking (SMT) experiments are well suited to the noninvasive study of membrane properties. In SMT experiments, the position of a single fluorescently labeled protein or lipid probe is followed optically as it moves within the membrane. If the motion of the probe is unhindered, then the atial trajectory of the molecule will follow two-dimensional Brownian motion. If the probe encounters a structure that in some way inhibits its movement, then the probe's trajectory will deviate from Brownian motion. It is likely that even if a certain type of lipid or protein partitions strongly into one nvironment, each individual lipid or protein will spend some fraction of its lifetime in the less favorable environment. Because SMT follows the motion of an individual probe over a large area (approximately 10 x 10 microm2), transitions between...Continue Reading

Citations

Feb 19, 2010·Analytical Chemistry·Samuel J LordW E Moerner
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michael A ThompsonW E Moerner
Jun 30, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jerry C ChangSandra J Rosenthal
Jan 27, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Mark J RichardsSusan Daniel
Sep 8, 2016·ACS Nano·Stephen A LeeJulie S Biteen
Dec 29, 2018·The Journal of Cell Biology·Brady D GouldenGerald R V Hammond
Sep 21, 2017·ACS Applied Materials & Interfaces·Han-Yuan LiuSusan Daniel

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