Single Muscle Fiber Proteomics Reveals Distinct Protein Changes in Slow and Fast Fibers during Muscle Atrophy

Journal of Proteome Research
Franziska LangMarcus Krüger

Abstract

Skeletal muscles are composed of heterogeneous collections of fibers with different metabolic profiles. With varied neuronal innervation and fiber-type compositions, each muscle fulfils specific functions and responds differently to stimuli and perturbations. We assessed individual fibers by mass spectrometry to dissect protein changes after loss of neuronal innervation due to section of the sciatic nerve in mice. This proteomics approach enabled us to quantify ∼600 proteins per individual soleus and EDL (extensor digitorum longus) muscle fiber. Expression of myosin heavy chain (MyHC) in individual fibers enabled clustering of specific fiber types; comparison of fibers from control and denervated muscles with the same MyHC expression revealed restricted regulation of a total of 240 proteins in type-I, -IIa, or -IIb fibers 7 days after denervation. The levels of several mitochondrial and proteasomal proteins were significantly altered, indicating rapid adaption of metabolic processes after denervation. Furthermore, we observed fiber-type-specific regulation of proteins involved in calcium ion binding and transport, such as troponins, parvalbumin, and ATP2A2, indicating marked remodeling of muscle contractility after denervation....Continue Reading

References

Jan 1, 1978·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D F Goldspink
Apr 15, 1976·The Biochemical Journal·D F Goldspink
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P VolpeJ Meldolesi
May 1, 1969·The American Journal of Physiology·A L Goldberg, H M Goodman
Jan 1, 1994·European Journal of Applied Physiology and Occupational Physiology·A C FryR S Staron
Aug 1, 1994·The American Journal of Physiology·L SchulteS Kandarian
Aug 26, 1998·The Journal of Physiology·A WindischT Lømo
Sep 22, 2000·Microscopy Research and Technique·D Pette, R S Staron
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·R G Shulman, D L Rothman
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jul 17, 2001·Current Opinion in Cell Biology·L Ellgaard, A Helenius
Dec 26, 2001·The Journal of Biological Chemistry·John G PastorinoJan B Hoek
Oct 17, 2002·Analytical Biochemistry·C PapageorgopoulosM Hellerstein
Feb 4, 2003·The Journal of Physiology·Henriette PilegaardP Darrell Neufer
Apr 1, 2003·IUBMB Life·Aldrin V GomesDanuta Szczesna-Cordary
May 21, 2003·The Journal of Experimental Biology·John E Wilson
Jul 17, 2003·Acta Physiologica Scandinavica·E E Spangenburg, F W Booth
Oct 2, 2003·Biology of the Cell·Onnik AgbulutGillian Butler-Browne
Dec 17, 2003·The Journal of Biological Chemistry·Belinda BullardWolfgang A Linke
Jan 14, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stewart H LeckerAlfred L Goldberg
Nov 23, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jennifer M SacheckAlfred L Goldberg
Jan 16, 2008·Physiological Reviews·D G AllenH Westerblad
Mar 21, 2008·The EMBO Journal·Julie Lagirand-CantaloubeSerge A Leibovitch
Jan 1, 2010·Current Pharmaceutical Design·Stefano Ciciliot, Stefano Schiaffino
Feb 9, 2010·Cell Death and Differentiation·C S LeeY-G Ko
Apr 2, 2010·BMB Reports·Sanjaya Kumar Sahoo, Do Han Kim
Apr 20, 2010·Biochemical and Biophysical Research Communications·Soon-Young Jung, Young-Gyu Ko
Jan 25, 2011·Journal of Proteome Research·Jürgen CoxMatthias Mann
Oct 21, 2011·Physiological Reviews·Stefano Schiaffino, Carlo Reggiani
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eric C CheungKaren H Vousden
Mar 16, 2013·Current Opinion in Clinical Nutrition and Metabolic Care·Yichen Wang, Jeffrey E Pessin

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Citations

Sep 20, 2020·Scientific Reports·Charlotte WarrenAmy E Vincent
Dec 13, 2019·Journal of Applied Physiology·Kaleen M LavinMarcas M Bamman
Oct 11, 2020·Nature Communications·Matthieu Dos SantosPascal Maire
Jan 7, 2021·Cells·Luisa GorzaMara Brancaccio
Dec 10, 2020·Dose-response : a Publication of International Hormesis Society·Wen-Qing XieShan Lv
May 6, 2021·American Journal of Physiology. Cell Physiology·Zachary A GrahamMarcas M Bamman
Jun 6, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Davide BindelliniVered Raz
Dec 11, 2021·Expert Review of Proteomics·Paul DowlingKay Ohlendieck

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