Single nanoparticle spectroscopy for real-time in vivo quantitative analysis of transport and toxicity of single nanoparticles in single embryos

The Analyst
Kerry J LeeXiao-Hong Nancy Xu

Abstract

Nanomaterials exhibit distinctive physicochemical properties and promise a wide range of applications from nanotechnology to nanomedicine, which raise serious concerns about their potential environmental impacts on ecosystems. Unlike any conventional chemicals, nanomaterials are highly heterogeneous, and their properties can alter over time. These unique characteristics underscore the importance of study of their properties and effects on living organisms in real time at single nanoparticle (NP) resolution. Here we report the development of single-NP plasmonic microscopy and spectroscopy (dark-field optical microscopy and spectroscopy, DFOMS) and ultrasensitive in vivo assay (cleavage-stage zebrafish embryos, critical aquatic species) to study transport and toxicity of single silver nanoparticles (Ag NPs, 95.4 ± 16.0 nm) on embryonic developments. We synthesized and characterized purified and stable (non-aggregation) Ag NPs, determined their sizes and doses (number), and their transport mechanisms and effects on embryonic development in vivo in real time at single-NP resolution. We found that single Ag NPs passively entered the embryos through their chorionic pores via random Brownian diffusion and stayed inside the embryos thr...Continue Reading

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Citations

Feb 16, 2013·Chemical Reviews·Anthony S StenderNing Fang
Sep 13, 2013·Chemical Research in Toxicology·Lauren M BrowningXiao-Hong Nancy Xu
Sep 24, 2013·PloS One·Junchao DuanZhiwei Sun
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Dec 1, 2014·The Science of the Total Environment·George MetreveliGabriele E Schaumann
Dec 18, 2013·Analytical Chemistry·Wei Wang, Nongjian Tao
Feb 25, 2018·Environmental Science and Pollution Research International·Bangeppagari ManjunathaSang Joon Lee
Apr 25, 2019·Annual Review of Analytical Chemistry·Fato Tano PatriceYi-Tao Long
Dec 31, 2019·Journal of Applied Toxicology : JAT·Changcun Bai, Meng Tang
Jun 17, 2020·Toxicology and Industrial Health·Zengjin Wang, Zhiping Wang
Apr 2, 2021·Nanoscale Advances·Preeyaporn SongkiatisakXiao-Hong Nancy Xu
Sep 22, 2016·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Lauren M BrowningXiao-Hong Nancy Xu
Aug 29, 2021·Ecotoxicology and Environmental Safety·Yaowen LiuKangJu Lee

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